製品の概要
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製品名Anti-CCBL2 antibody
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製品の詳細Rabbit polyclonal to CCBL2
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由来種Rabbit
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アプリケーション適用あり: WB, ELISAmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
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免疫原
A region within synthetic peptide: LKFTNAKRIE GLDSNVWIEF TKLAADPSVV NLGQGFPDIS PPTYVKEELS, corresponding to N terminal amino acids 37-86 of Human CCBL2
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ポジティブ･コントロール
- Jurkat cell lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab56028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 2.5 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| ELISA | Use at an assay dependent concentration. ELISA titre using peptide based assay: 1/312500. |
ターゲット情報
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関連性CCBL2 (Cysteine-S-conjugate beta-lyase 2) is an aminotransferase that transaminates kynurenine to form kynurenic acid. Kynurenic acid is a metabolite of tryptophan. CCBL2 may catalyze the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond. Multiple alternatively spliced transcript variants that encode different proteins have been described for this gene. One of the transcripts encodes a predicted protein that has sequence identity to the protein encoded by the RNA binding motif protein, X-linked gene (RBMX).
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参照データベース
- Entrez Gene: 479959 Dog
- Entrez Gene: 56267 Human
- Entrez Gene: 229905 Mouse
- Entrez Gene: 393315 Zebrafish
- Omim: 610656 Human
- SwissProt: Q6YP21 Human
- SwissProt: Q71RI9 Mouse
- SwissProt: Q58FK9 Rat
see all -
別名
- CCBL 2 antibody
- Cysteine conjugate beta lyase 2 antibody
- Cysteine S conjugate beta lyase 2 antibody
see all
画像
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Western blot - CCBL2 antibody (ab56028)Anti-CCBL2 antibody (ab56028) at 2.5 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 51 kDa
Observed band size: 43 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
プロトコール
参考文献
ab56028 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"