製品の概要

  • 製品名Anti-cbx7 antibody - ChIP Grade
    cbx7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to cbx7 - ChIP Grade
  • アプリケーション適用あり: ICC/IF, ChIP, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human cbx7.

  • ポジティブ・コントロール
    • ab21873 gave a positive result in the following nuclear lysates: Hela, Jurkat, HepG2. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal protate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa).
IHC-P Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性Control of cell proliferation by Polycomb group proteins (PcG) is an important facet of cellular homeostasis and its disruption can promote tumorigenesis. CBX7 is a novel PcG protein which is found to control the growth of normal cells. CBX7 is a gene that encodes a Polycomb protein, as shown by sequence homology, its interaction with Ring1 and its localization to nuclear Polycomb bodies. CBX7 extends the lifespan of a wide range of normal human cells and immortalizes mouse fibroblasts by downregulating expression of the Ink4a/Arf locus. CBX7 does not inter-function or colocalize with Bmi1, and both can exert their actions independently of each other as shown by reverse genetics. CBX7 was highly expressed in three different prostate cancer cell lines and present at elevated levels in normal prostate. (Gil et al., 2004).
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • Cbx 7 antibody
    • CBX7 antibody
    • Chromobox homolog 7 antibody
    • Chromobox protein homolog 7 antibody

Anti-cbx7 antibody - ChIP Grade 画像

  • All lanes : Anti-cbx7 antibody - ChIP Grade (ab21873) at 1 µg

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)
    Lane 3 : HepG2 nuclear extract lysate (ab14660)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ab21873 (1/1000) staining cbx7 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

  • IHC image of cbx7 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21873, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-cbx7 antibody - ChIP Grade (ab21873) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : Jurkat nuclear extract lysate (ab14844)
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : HepG2 nuclear extract lysate (ab14660)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,70 kDa. We are unsure as to the identity of these extra bands.

Anti-cbx7 antibody - ChIP Grade (ab21873) 使用論文

This product has been referenced in:
  • Pallante P  et al. CBX7 modulates the expression of genes critical for cancer progression. PLoS One 9:e98295 (2014). WB, ChIP ; Human . Read more (PubMed: 24865347) »
  • Blackledge NP  et al. Variant PRC1 complex-dependent H2A ubiquitylation drives PRC2 recruitment and polycomb domain formation. Cell 157:1445-59 (2014). ChIP . Read more (PubMed: 24856970) »

See all 12 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skin)
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Ahmar Aziz

Verified customer

投稿 Jul 08 2016

Application ChIP
Sample Mouse Cell lysate - nuclear (mES E14)
Negative control RA treated cells
Specification mES E14
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: PAF
Positive control Cbx7 Overexpressing Cells
Username

Abcam user community

Verified customer

投稿 Aug 12 2015

Application Western blot
Sample Mouse Cell lysate - whole cell (mES E14)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Treatment Retinoic Acid
Specification mES E14
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 12 2015

Following up my previous message, the antibody ab123492 has not been tested for zebrafish reactivity and is unlikely to react, based on alignment of the immunogen with the zebrafish sequence you provided.
Please let us know if you have any other ...

Read More

The immunogen is derived from the human CBX7 at the following UniProt entry URL:

http://www.uniprot.org/uniprot/O95931 .

Alignment of the immunogen with the sequence provided gave poor results.
Unfortunately, alignments of th...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Apr 18 2012

I have issued a free of charge replacement with the order number 1042072.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that typically our Abpromise guarantee hold...

Read More

Thanks for your reply.

You are correct that lot GR21097-1 is a new lot we have in stock. We are not able to stock small sample aliqots of our antibodies unfortunately. However, since a vial of ab21873 you previously purchased is not working a...

Read More

Thank you for your enquiry. I am sorry to hear of the trouble you are having with lot 937205 in ChIP. I looked in our records and there was one other complaint of a similar nature regarding ChIP with this lot of ab21873. We do have a new lot in stock n...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Specification mouse embryonic stem cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step Real-time PCR
Positive control Polycomb targets
Negative control Ensa, B2M, RPS14 genes
Username

Dr. Ligia Tavares

Verified customer

投稿 Sep 23 2011

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"