製品の概要

  • 製品名Anti-CBX4 antibody
    CBX4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CBX4
  • 特異性In immunofluoresence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localisation of PC2. The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see picture). It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody also reacts with another ubiquitous highly expressed protein.
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    GLQDSSTDNRAKLC

    , corresponding to amino acids 95 - 107 of Human PC2.

  • 特記事項


    PC2 and other members of the Drosophila Polycomb group gene family are part of a cellular memory system that is responsible for the inheritance of gene activity by progeny cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 82 kDa (predicted molecular weight: 61 kDa).

ターゲット情報

  • 機能E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • 組織特異性Ubiquitous.
  • パスウェイProtein modification; protein sumoylation.
  • 配列類似性Contains 1 chromo domain.
  • 翻訳後修飾Phosphorylated on Thr-497 by HIPK2 upon DNA damage; which enhances E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CBX 4 antibody
    • CBX4 antibody
    • Cbx4 chromobox homolog 4 (Drosophila Pc class) antibody
    • CBX4_HUMAN antibody
    • Chromobox homolog 4 (Pc class homolog, Drosophila) antibody
    • Chromobox homolog 4 antibody
    • Chromobox protein homolog 4 antibody
    • E3 SUMO protein ligase CBX 4 antibody
    • E3 SUMO protein ligase CBX4 antibody
    • E3 SUMO-protein ligase CBX4 antibody
    • hPc 2 antibody
    • hPc2 antibody
    • NBP 16 antibody
    • NBP16 antibody
    • NS5ATP1 binding protein 16 antibody
    • Pc 2 antibody
    • Pc class 2 homolog antibody
    • Pc class homolog antibody
    • Pc class homolog Drosophila antibody
    • PC2 antibody
    • Polycomb 2 homolog antibody
    see all

Anti-CBX4 antibody 画像

  • Immunofluorescent imaging of human cells (U2OS) with ab4189 reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localisation of PC2. This corresponds to results seen in Satijn et al.

    IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/200 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees C. Nuclei counterstained with Hoechst stain (blue).

  • All lanes : Anti-CBX4 antibody (ab4189)

    Lane 1 : HEK 293 lysate over-expressing DDDDK tagged hPC2
    Lane 2 : HEK 293 lysate


    Predicted band size : 61 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa (possible non-specific secondary antibody binding),49 kDa (possible non-specific secondary antibody binding),65 kDa (possible non-specific secondary antibody binding).

    This image is courtesy of Dr Ari Melnick, Albert Einstein College of Medicine

    ab4189 specifically recognises over expressed DDDDK tagged hPC2 in HEK293 cells (lane1). The lower bands are assigned to degradation products.

Anti-CBX4 antibody (ab4189) 使用論文

This product has been referenced in:
  • MacPherson MJ  et al. The CTCF insulator protein is posttranslationally modified by SUMO. Mol Cell Biol 29:714-25 (2009). ICC/IF ; Human . Read more (PubMed: 19029252) »

See 1 Publication for this product

Product Wall

I'm sorry to hear you are having problems with ab4189, I would like to suggest a few modifications to your protocol: -try a different fixation step, I would suggest a 10min acetone or methanol (ice cold) fixation, it can be a very good fixative in ...

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