製品の概要
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製品名Anti-CBX2 antibody
CBX2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to CBX2
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由来種Rabbit
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アプリケーション適用あり: IP, WBmore details
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種交差性交差種: Mouse, Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human cbx2.
(Peptide available as ab91615.)
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ポジティブ･コントロール
- This antibody gave a positive signal in the following lysates: JEG3 whole cell; HUVEC whole cell; HEK293 whole cell; MEL-1 whole cell; MEL-2 whole cell; HeLa nuclear; NIH3T3 whole cell; human testis tissue.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab80044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa). |
ターゲット情報
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機能Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Involved in sexual development, acting as activator of NR5A1 expression.
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関連疾患Defects in CBX2 are the cause of 46,XY sex reversal type 5 (SRXY5) [MIM:613080]. It is a disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females.
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配列類似性Contains 1 A.T hook DNA-binding domain.
Contains 1 chromo domain. -
細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 84733 Human
- Entrez Gene: 12416 Mouse
- Omim: 602770 Human
- SwissProt: Q14781 Human
- SwissProt: P30658 Mouse
- Unigene: 368410 Human
- Unigene: 14547 Mouse
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別名
- Cbx 2 antibody
- CBX2 antibody
- CBX2_HUMAN antibody
see all
画像
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Western blot - Anti-CBX2 antibody (ab80044)All lanes : Anti-CBX2 antibody (ab80044) at 1 µg/ml
Lane 1 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lane 5 : MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196)
Lane 6 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 52 kDa (why is the actual band size different from the predicted?)
Additional bands at: 36 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Western blot - Anti-CBX2 antibody (ab80044)All lanes : Anti-CBX2 antibody (ab80044) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 8 minutes -
Immunoprecipitation - Anti-CBX2 antibody (ab80044)cbx2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to cbx2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab80044.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 52kDa: cbx2.
参考文献
This product has been referenced in:
- Zhen CY et al. Cbx2 stably associates with mitotic chromosomes via a PRC2- or PRC1-independent mechanism and is needed for recruiting PRC1 complex to mitotic chromosomes. Mol Biol Cell 25:3726-39 (2014). WB ; Mouse . Read more (PubMed: 25232004) »
- Klauke K et al. Polycomb Cbx family members mediate the balance between haematopoietic stem cell self-renewal and differentiation. Nat Cell Biol 15:353-62 (2013). WB . Read more (PubMed: 23502315) »
レビューと Q&A
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