Anti-CBX1 / HP1 beta 抗体 [MAC353] - ChIP Grade (ab10811)


  • 製品名Anti-CBX1 / HP1 beta antibody [MAC353] - ChIP Grade
    CBX1 / HP1 beta 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [MAC353] to CBX1 / HP1 beta - ChIP Grade
  • 特異性Ab10811 recognises the M31 molecule in mouse and the homologous HP1 HS beta molecule in man.
  • アプリケーション適用あり: IHC-Fr, ELISA, WB, ICC/IF, ChIP, IPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    71 amino acid fusion protein corresponding to the c-terminal region of M31 (human/mouse).

  • ポジティブ・コントロール
    • Murine and human nuclear extracts.



Our Abpromise guarantee covers the use of ab10811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/1 - 1/50.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 26 kDa (predicted molecular weight: 22.2 kDa).
ICC/IF Use at an assay dependent dilution.
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent dilution.


  • 機能Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.
  • 組織特異性Expressed in all adult and embryonic tissues.
  • 配列類似性Contains 2 chromo domains.
  • 翻訳後修飾Not phosphorylated.
  • 細胞内局在Nucleus. Unassociated with chromosomes during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CBX 1 antibody
    • CBX antibody
    • Cbx1 antibody
    • CBX1_HUMAN antibody
    • Chromobox 1 antibody
    • Chromobox homolog 1 (HP1 beta homolog Drosophila ) antibody
    • chromobox homolog 1 antibody
    • Chromobox protein homolog 1 antibody
    • Drosophila HP1 beta antibody
    • Heterochromatin protein 1 beta antibody
    • Heterochromatin protein 1 homolog beta antibody
    • Heterochromatin protein p25 antibody
    • heterochromatin protein p25 beta antibody
    • HP1 beta antibody
    • HP1 beta homolog antibody
    • HP1 beta homolog Drosophila antibody
    • HP1, Drosophila. homolog of, beta antibody
    • HP1beta antibody
    • HP1Hs beta antibody
    • HP1Hsbeta antibody
    • Human heterochromatin protein p25 mRNA complete cds antibody
    • M31 antibody
    • MOD 1 antibody
    • MOD1 antibody
    • Modifier 1 protein antibody
    • p25beta antibody
    see all

Anti-CBX1 / HP1 beta antibody [MAC353] - ChIP Grade 画像

  • Predicted band size : 22.2 kDa

    Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: CBX1 knockout HAP1 cell lysate (40 µg)
    Lane 3: MCF7 cell lysate (40 µg)
    Lane 4: A431 cell lysate (40 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab10811 observed at 26 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab10811 was shown to specifically react with CBX1 / HP1 beta when CBX1 / HP1 beta knockout samples were used. Wild-type and CBX1 / HP1 beta knockout samples were subjected to SDS-PAGE. Ab10811 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

  • HeLA cells were stained with ab10811 in panel one. In panel two they were stained with ab10811 (green), DAPI (blue) and SH-CREST (red), which stains the centromeres. Fix 30 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with 1 mg/ml Na borohydride or 100 mM ammonium chloride in PEM. Permeablize 30 minutes with 0.5% TX-100 in PEM. Block 30 minutes in 5% milk in TBST. Primary antibody incubated overnight at 4oC diluted 1/100 in 5% milk in TBST. Secondary antibody incubated 1 hour at RT diluted in 5% milk in TBST. Post-fix 20 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with ammonium chloride in PEM. Counterstain with DAPI in TBST. Mount with ProLong Gold antifade reagent from Invitrogen. Notes: Ample washing between each step. TBST = Tris buffered saline + 0.1% Tween. PEM = 80 mM K-PIPES, pH 6.8, 5 mM EGTA, 2 mM MgCl2.
  • Immunofluorescence analysis of mouse erythroleukemia cell nuclei, staining CBX1 / HP1 beta in heterochromatin, with ab10811.

    Cells were fixed with paraformaldehyde, permeabilized using Triton X-100 and blocked for 30 min with 2.5% BSA. Cells were incubated with primary antibody (1/50) before incubating with a Cy3-conjugated goat anti-rat IgG to detect staining.

Anti-CBX1 / HP1 beta antibody [MAC353] - ChIP Grade (ab10811) 使用論文

This product has been referenced in:
  • Vaskova EA  et al. Dynamics of the two heterochromatin types during imprinted X chromosome inactivation in vole Microtus levis. PLoS One 9:e88256 (2014). ICC/IF . Read more (PubMed: 24505450) »
  • Tretiakova MS  et al. Heterochromatin protein 1 expression is reduced in human thyroid malignancy. Lab Invest 94:788-95 (2014). Read more (PubMed: 24840329) »

See all 6 Publications for this product

Product Wall

Thank you for contacting us.

For ChIP, we recommend to optimise the working concentration according to sample and experiment. At the starting point, you could try using 1:50 dilution.

I hope this information is helpful ...

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Thank you for contacting us with your questions. Since the immunogen of the antibody was taken from the C-terminus, and threonine 51 is much closer to the N-terminus, the antibody will detect the total CBX1 protein. The antibody will recognize CBX1 ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Colorectal Cancer Cells)
Specification Colorectal Cancer Cells
Fixative Formaldehyde
Permeabilization Yes - triton x-100
Blocking step Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Abcam user community

Verified customer

投稿 Jan 21 2010

I'm glad you like the mug! We are unable to provide a concentration for ab10811 as it is in an unpurified form, tissue culture supernatant, and we cannot guarantee the concentration for unpurified material. I recommend starting at a dilution of 1:1000 ...

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