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71 amino acid fusion protein corresponding to the c-terminal region of M31 (human/mouse).
Our Abpromise guarantee covers the use of ab10811 in the following tested applications.
|IHC-Fr||1/1 - 1/50.|
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Detects a band of approximately 26 kDa (predicted molecular weight: 22.2 kDa).|
|ICC/IF||Use at an assay dependent dilution.|
|ChIP||Use at an assay dependent concentration.|
|IP||Use at an assay dependent dilution.|
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: CBX1 knockout HAP1 cell lysate (40 µg)
Lane 3: MCF7 cell lysate (40 µg)
Lane 4: A431 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab10811 observed at 26 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab10811 was shown to specifically react with CBX1 / HP1 beta when CBX1 / HP1 beta knockout samples were used. Wild-type and CBX1 / HP1 beta knockout samples were subjected to SDS-PAGE. Ab10811 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.