製品の概要

  • 製品名Anti-CBLN1 antibody
    CBLN1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CBLN1
  • 特異性ab92526 detects endogenous levels of total CBLN1 protein.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of Human CBLN1.

  • ポジティブ・コントロール
    • HUVEC cell extracts.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab92526 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab92526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報ELISA: 1/40000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 21 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the formation and maintenance of parallel fiber and Purkinje cell synapses. When parallel fibers make contact with Purkinje spines, CBLN1 interaction with GRID2 triggers the recruitment of NRXN1 and secretory vesicles to the sites of contact. NRXN1-CBLN1-GRID2 signaling induces presynaptic morphological changes, which may further accumulate pre- and postsynaptic components to promote bidirectional maturation of parallel fiber - Purkinje cell functionnally active synapses by a positive feedback mechanism. Required for CBLN3 export from the endoplasmic reticulum and secretion.
      The cerebellin peptide exerts neuromodulatory functions. Directly stimulates norepinephrine release via the adenylate cyclase/PKA-dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release.
    • 組織特異性In the Purkinje cells postsynaptic structures. In the cerebellum, cerebellin is much less abundant than [des-Ser1]-cerebellin.
    • 配列類似性Contains 1 C1q domain.
    • 発生段階Low at birth, the cerebellin concentration increases between day 5 and 15, and reaches peak values between day 21 and 56.
    • 翻訳後修飾The proteolytic processing to yield cerebellin seems to occur either prior to the secretion by presynaptic neurons and subsequent oligomerization or in some other location after release of the mature protein.
    • 細胞内局在Secreted. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • [des-Ser1]-cerebellin antibody
      • CBLN 1 antibody
      • CBLN1 antibody
      • CBLN1_HUMAN antibody
      • CER antibody
      • Cerebellin 1 antibody
      • Cerebellin 1 precursor antibody
      • Cerebellin1 antibody
      • Precerebellin antibody
      see all

    Anti-CBLN1 antibody 画像

    • All lanes : Anti-CBLN1 antibody (ab92526) at 1/500 dilution

      Lane 1 : HUVEC cell extracts
      Lane 2 : HUVEC cell extracts with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 21 kDa

    Anti-CBLN1 antibody (ab92526) 使用論文

    ab92526 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab92526.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"