製品の概要

  • 製品名Anti-CBLL1 antibody
    CBLL1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CBLL1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: FTQPGGMSPG IWPAPRGPPP PPRLQGPPSQ TPLPGPHHPD QTRYRPYYQ, corresponding to C terminal amino acids 443-491 of Human CBLL1

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

ターゲット情報

  • 機能Promotes ubiquitination of tyrosine-phosphorylated CDH1, thus targeting CDH1 for endocytosis and degradation.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 C2H2-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Information by UniProt
  • 参照データベース
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Anti-CBLL1 antibody 画像

  • Lane 1 : MW marker
    Lane 2 : Anti-CBLL1 antibody (ab50993) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 55 kDa

Anti-CBLL1 antibody (ab50993) 使用論文

ab50993 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (gel 8%)
Sample Human Cell lysate - whole cell (cell line MDCK)
Specification cell line MDCK
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 27°C
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宣 若橋

Verified customer

投稿 Jul 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"