製品の概要

  • 製品名Anti-CBLB antibody [246C5a]
    CBLB 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [246C5a] to CBLB
  • アプリケーション適用あり: IHC-P, Dot blot, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant C terminal fragment of Human CBLB.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 109 kDa.

ターゲット情報

  • 関連性Function: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. Tissue specificity: Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle. Present in lymphocytes (at protein level). Pathway: Protein modification; protein ubiquitination. Similarity: Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain. Contains 1 RING-type zinc finger. Contains 1 UBA domain. Domain: The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain. The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. The UBA domain interacts with poly-ubiquitinated proteins. PTM: Phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation. Auto-ubiquitinated upon EGF-mediated cell activation or upon T-cell costimulation by CD28; which promotes proteasomal degradation.
  • 細胞内局在Cytoplasmic and Nuclear
  • 参照データベース
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Anti-CBLB antibody [246C5a] 画像

  • Anti-CBLB antibody [246C5a] (ab54362) + immunizing recombinant protein

    Predicted band size : 109 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab54362 staining in humn T-cell lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab54362, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CBLB antibody [246C5a] (ab54362) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 1e+007 cells
Specification mouse embryonic stem cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Miss. Fabiana Luise

Verified customer

投稿 Nov 17 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"