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RabMAb

Anti-CBL (phospho Y774) 抗体 [E160] (ab32446)

Submit an Abreview Q&A (1)Specific References (2)

製品の概要

  • 製品名
    Anti-CBL (phospho Y774) antibody [E160]
    CBL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E160] to CBL (phospho Y774)
  • 由来種
    Rabbit
  • 特異性
    The antibody detects Cbl phosphorylated on Tyrosine 774.
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Tyr774 of human Cbl.

  • ポジティブ･コントロール
    • Jurkat cell lysate and human cervical carcinoma tissue.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/25.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/20.

ターゲット情報

  • 機能
    Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 関連疾患
    Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.
  • 配列類似性
    Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
    Contains 1 RING-type zinc finger.
    Contains 1 UBA domain.
  • ドメイン
    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
    The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
  • 翻訳後修飾
    Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
    • 別名
      • 4732447J05Rik antibody
      • C CBL antibody
      • Cas Br M (murine) ecotropic retroviral transforming sequence antibody
      • Casitas B lineage lymphoma proto oncogene antibody
      • Casitas B-lineage lymphoma proto-oncogene antibody
      • CBL 2 antibody
      • cbl antibody
      • CBL_HUMAN antibody
      • CBL2 antibody
      • E3 ubiquitin protein ligase CBL antibody
      • E3 ubiquitin-protein ligase CBL antibody
      • Oncogene CBL2 antibody
      • Proto oncogene c CBL antibody
      • Proto-oncogene c-CBL antibody
      • RGD1561386 antibody
      • RING finger protein 55 antibody
      • RNF55 antibody
      • Signal transduction protein CBL antibody
      see all

    画像

    • Western blot - CBL (phospho Y774) antibody [E160] (ab32446)
      Western blot - CBL (phospho Y774) antibody [E160] (ab32446)
      All lanes : Anti-CBL (phospho Y774) antibody [E160] (ab32446) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat cell lysate + Pervanadate

      Predicted band size: 99 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL (phospho Y774) antibody [E160] (ab32446)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL (phospho Y774) antibody [E160] (ab32446)
      Immunohistochemical analysis of human cervical carcinoma using ab32446 at 1/25 dilution.
    • Flow Cytometry / FACS - CBL (phospho Y774) antibody [E160] (ab32446)
      Flow Cytometry / FACS - CBL (phospho Y774) antibody [E160] (ab32446)
      Flow cytometric analysis of permeabilized Jurkat cells, untreated (green) or Pervanadate-treated (red), using ab32446 at 1/20 dilution.

    参考文献

    This product has been referenced in:
    • Wakasaki T  et al. A critical role of c-Cbl-interacting protein of 85 kDa in the development and progression of head and neck squamous cell carcinomas through the ras-ERK pathway. Neoplasia 12:789-96 (2010). WB . Read more (PubMed: 20927317) »
    • Cai J  et al. Diacylglycerol kinase delta and protein kinase C(alpha) modulate epidermal growth factor receptor abundance and degradation through ubiquitin-specific protease 8. J Biol Chem 285:6952-9 (2010). Read more (PubMed: 20064931) »

    See all 2 Publications for this product

    ab32446 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    質問を送る

    1 Q&A

    10
    Votes: Highest First

    How were the Jurkat cells in the WB image treated?

    Jurkat cells were starved overnight, then treated with 50mM Pervanadate for 10min.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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