製品の概要

  • 製品名Anti-CBL (phospho Y774) antibody
    CBL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CBL (phospho Y774)
  • 特異性Recognises CBL when phosphorylated at these sites: Human: Tyr774; Mouse: Tyr780; Rat: Tyr888
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human CBL around the phosphorylation site of tyrosine 774 (D-G-YP-D-V).

  • ポジティブ・コントロール
    • HeLa cells extracts Human breast carcinoma tissue
  • 特記事項Stable at -20°C for 1 year

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 99 kDa).
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
  • パスウェイProtein modification; protein ubiquitination.
  • 関連疾患Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.
  • 配列類似性Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
    Contains 1 RING-type zinc finger.
    Contains 1 UBA domain.
  • ドメインThe RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
    The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
  • 翻訳後修飾Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
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Anti-CBL (phospho Y774) antibody 画像

  • All lanes : Anti-CBL (phospho Y774) antibody (ab58505) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells, treated with EGF (200ng/ml, 30mins)
    Lane 2 : Extracts from HeLa cells, treated with EGF (200ng/ml, 30mins), plus immunising peptide


    Predicted band size : 99 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • ab58505 at a 1/50 dilution staining CBL (phosphorylated at tyrosine 774) in Human breast carcinoma tissue.
  • ab58505, at a 1/50 dilution, plus immunising peptide, staining CBL (phosphorylated at tyrosine 774) in Human breast carcinoma tissue.

Anti-CBL (phospho Y774) antibody (ab58505) 使用論文

ab58505 has not yet been referenced specifically in any publications.

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