製品の概要

  • 製品名Anti-CBL (phospho Y700) antibody [EP2225Y]
    CBL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2225Y] to CBL (phospho Y700)
  • 特異性ab76002 detects CBL phosphorylated on tyrosine 700.
  • アプリケーション適用あり: WB, IP, Flow Cyt, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding tyrosine 700 of Human CBL

  • ポジティブ・コントロール
    • Jurkat cell lysate, untreated or treated with pervanadate; permeabilized Jurkat cells, untreated or treated with pervanadate.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab76002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa).
IP 1/40.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 22615563
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
    • パスウェイProtein modification; protein ubiquitination.
    • 関連疾患Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.
    • 配列類似性Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
      Contains 1 RING-type zinc finger.
      Contains 1 UBA domain.
    • ドメインThe RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
      The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
    • 翻訳後修飾Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
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    Anti-CBL (phospho Y700) antibody [EP2225Y] 画像

    • Flow cytometric analysis of ab76002 at 1/50 dilution staining permeabilized Jurkat cells, untreated (green) or pervanadate-treated (red) and pervanadate-treated Jurkat cells preincubated with immunising phospho-peptide (blue) or non-phospho peptide (orange).
    • All lanes : Anti-CBL (phospho Y700) antibody [EP2225Y] (ab76002) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate, untreated
      Lane 2 : Jurkat cell lysate, treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 99 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)

    Anti-CBL (phospho Y700) antibody [EP2225Y] (ab76002) 使用論文

    This product has been referenced in:
    • Greene W  et al. The ubiquitin/proteasome system mediates entry and endosomal trafficking of Kaposi's sarcoma-associated herpesvirus in endothelial cells. PLoS Pathog 8:e1002703 (2012). ICC/IF . Read more (PubMed: 22615563) »

    See 1 Publication for this product

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