製品の概要

  • 製品名Anti-Cbl-c antibody
    Cbl-c 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cbl-c
  • 特異性No reactivity is expected with Cbl-a or Cbl-b.
  • アプリケーション適用あり: ELISA, WB, IP, ICC/IFmore details
  • 種交差性

    交差が予測される動物種: Mouse, Rat, Human, Chimpanzee
  • 免疫原

    Synthetic peptide:

    PRKPRNAQPKVRLLK

    , corresponding to amino acids 444-458 of Human Cbl-c

  • ポジティブ・コントロール
    • Hek 293T cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The product was affinity purified from monospecific antiserum by immunoaffinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab34750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000 - 1/50000.
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 関連性Cblc belongs to a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cblc is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitous). Cblc, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Cblc knock out mice show no obvious phenotype. Thus, the physiological role of Cblc is not known.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • Cas Br M (murine) ecotropic retroviral transforming sequence c antibody
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    • Casitas B lineage lymphoma c antibody
    • CBL 3 antibody
    • Cbl proto-oncogene C, E3 ubiquitin protein ligase antibody
    • Cbl proto-oncogene, E3 ubiquitin protein ligase C antibody
    • CBL SL antibody
    • E3 ubiquitin-protein ligase CBL-C antibody
    • RING finger protein 57 antibody
    • RNF57 antibody
    • SH3 binding protein CBL C antibody
    • SH3-binding protein CBL-3 antibody
    • Signal transduction protein CBL C antibody
    see all

Anti-Cbl-c antibody 画像

  • All lanes : Anti-Cbl-c antibody (ab34750) at 1/500 dilution

    Lane 1 : Hek 293T cell lysate transfected with empty vector
    Lane 2 : Hek 293T cell lysate transfected with Cbl-c.
    Lane 3 : Hek 293T cell lysate transfected with empty vector
    Lane 4 : Hek 293T cell lysate transfected with Cbl-c.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Image courtesy of Human Protein Atlas
    ab34750 (1/500) showing strong nuclear and cytoplasmic stainning of cells of the kidney tubuli. Paraffin embedded human kidney tissue was incubated with ab123 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

Anti-Cbl-c antibody (ab34750) 使用論文

ab34750 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (7.5)
Sample Mouse Tissue lysate - whole (Intestine Brain mammary gland)
Specification Intestine Brain mammary gland
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Neha Zutshi

Verified customer

投稿 Jan 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"