製品の概要

  • 製品名
    Anti-CBL antibody [YE323]
    CBL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [YE323] to CBL
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC, WB, Flow Cyt, IPmore details
    適用なし: IHC
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chicken
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CBL aa 850 to the C-terminus (C terminal).
    Database link: P22681

  • エピトープ
    ab32027 reacts with an epitope located in the C terminal region of CBL.
  • ポジティブ・コントロール
    • Jurkat cell lysates, Hela Cells.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/100.
WB 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa).
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/100.
  • 追加情報
    Is unsuitable for IHC.
  • ターゲット情報

    • 機能
      Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 関連疾患
      Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.
    • 配列類似性
      Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
      Contains 1 RING-type zinc finger.
      Contains 1 UBA domain.
    • ドメイン
      The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
      The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
    • 翻訳後修飾
      Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 4732447J05Rik antibody
      • C CBL antibody
      • Cas Br M (murine) ecotropic retroviral transforming sequence antibody
      • Casitas B lineage lymphoma proto oncogene antibody
      • Casitas B-lineage lymphoma proto-oncogene antibody
      • CBL 2 antibody
      • cbl antibody
      • CBL_HUMAN antibody
      • CBL2 antibody
      • E3 ubiquitin protein ligase CBL antibody
      • E3 ubiquitin-protein ligase CBL antibody
      • Oncogene CBL2 antibody
      • Proto oncogene c CBL antibody
      • Proto-oncogene c-CBL antibody
      • RGD1561386 antibody
      • RING finger protein 55 antibody
      • RNF55 antibody
      • Signal transduction protein CBL antibody
      see all

    画像

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: CBL knockout HAP1 whole cell lysate (20 µg)
      Lane 3: THP1 whole cell lysate (20 µg)
      Lane 4: Raji whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab32027 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab32027 was shown to specifically react with CBL in wild-type HAP1 cells as signal was lost in CBL knockout cells. Wild-type and CBL knockout samples were subjected to SDS-PAGE. ab32027 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Anti-CBL antibody [YE323] (ab32027) at 1/5000 dilution + Jurkat cell lysates

      Predicted band size: 99 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)

    • Immunofluorescent staining of HeLa cells using 1/100 anti-Cbl (C-term) ab32027.

    参考文献

    This product has been referenced in:
    • Peng L  et al. Huntingtin-Interacting Protein 1-Related Protein Plays a Critical Role in Dendritic Development and Excitatory Synapse Formation in Hippocampal Neurons. Front Mol Neurosci 10:186 (2017). WB, ICC ; Rat . Read more (PubMed: 28663723) »
    • Walsh AM & Lazzara MJ Regulation of EGFR trafficking and cell signaling by Sprouty2 and MIG6 in lung cancer cells. J Cell Sci 126:4339-48 (2013). WB ; Human . Read more (PubMed: 23868981) »

    See all 2 Publications for this product

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab32027.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録