製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CBFb
  • 特異性
    This antibody detects CBFb.
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Zebrafish
    交差が予測される動物種: Chicken, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    MPRVVPDQRSKFENEEFFRK

    , corresponding to amino acids 1-20 of Human CBFb.

  • ポジティブ・コントロール
    • HeLa whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: PBS, 1mg/ml BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500. PubMed: 24648201
WB 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

ターゲット情報

  • 機能
    CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.
  • 関連疾患
    Note=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11.
  • 配列類似性
    Belongs to the CBF-beta family.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CBF b antibody
    • CBF beta antibody
    • CBF-beta antibody
    • CBFB antibody
    • CBFbeta antibody
    • Core binding factor beta subunit antibody
    • core binding factor subunit beta antibody
    • Core-binding factor subunit beta antibody
    • PEA 2 antibody
    • PEA2 antibody
    • PEA2 beta antibody
    • PEA2-beta antibody
    • PEA2beta antibody
    • PEBB_HUMAN antibody
    • PEBP 2B antibody
    • PEBP2 beta antibody
    • PEBP2-beta antibody
    • PEBP2B antibody
    • PEBP2beta antibody
    • Polyomavirus enhancer binding protein 2 beta subunit antibody
    • Polyomavirus enhancer-binding protein 2 beta subunit antibody
    • SL3 3 enhancer factor 1 beta subunit antibody
    • SL3 3 enhancer factor 1 subunit beta antibody
    • SL3-3 enhancer factor 1 subunit beta antibody
    • SL3/AKV core binding factor beta subunit antibody
    • SL3/AKV core-binding factor beta subunit antibody
    see all

参考文献

This product has been referenced in:
  • Cieslak A  et al. RUNX1-dependent RAG1 deposition instigates human TCR-d locus rearrangement. J Exp Med 211:1821-32 (2014). WB, IP ; Human . Read more (PubMed: 25135298) »
  • Lopez-Camacho C  et al. Core binding factor ß (CBFß) is retained in the midbody during cytokinesis. J Cell Physiol 229:1466-74 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24648201) »

See all 9 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic stem cells Strain 129)
Loading amount
35 µg
Specification
Mouse Embryonic stem cells Strain 129
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Oct 04 2010

Application
Western blot
Sample
Rat Cell lysate - whole cell (UMR-106 OSTEOSARCOMA)
Loading amount
50 µg
Specification
UMR-106 OSTEOSARCOMA
Gel Running Conditions
Reduced Denaturing (10% PAGE SDS)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Cesar Camacho

Verified customer

投稿 May 06 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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