製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to CBF
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Full length human CBF protein (NP_005751.2).

  • ポジティブ・コントロール
    • WB: NIH/3T3 cell lysate; K-562 cell lysate; CBF transfected 293T cell lysate ICC/IF: HeLa cell

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 121 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

画像

  • Anti-CBF antibody (ab89034) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg

    Predicted band size : 121 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • Anti-CBF antibody (ab89034) at 1 µg/ml + K-562 cell lysate at 50 µg

    Predicted band size : 121 kDa
    Observed band size : 131 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CBF antibody (ab89034) at 1 µg/ml

    Lane 1 : CEBPZ transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 121 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
  • ab89034, at 10 µg/ml, staining CBF in Hela cell by Immunofluorescence.

参考文献

ab89034 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab89034.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録