Anti-Caveolin-2 (phospho Y27) 抗体 [EP2221Y] (ab76405)

製品の概要

  • 製品名
    Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y]
    Caveolin-2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2221Y] to Caveolin-2 (phospho Y27)
  • 特異性
    This antibody detects Caveolin-2 phosphorylated at tyrosine 27.
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC/IF or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caveolin-2.
    Database link: P51636

  • ポジティブ・コントロール
    • Hela cell lysates
  • 特記事項

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 18 kDa.
IP 1/40.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • ターゲット情報

    • 機能
      May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
    • 組織特異性
      Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
    • 配列類似性
      Belongs to the caveolin family.
    • 翻訳後修飾
      Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
    • 細胞内局在
      Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CAV antibody
      • CAV2 antibody
      • CAV2_HUMAN antibody
      • Caveolae protein 20 kD antibody
      • Caveolin 2 antibody
      • Caveolin 2 isoform a and b antibody
      • Caveolin-2 antibody
      • MGC12294 antibody
      • OTTHUMP00000025032 antibody
      • OTTHUMP00000195982 antibody
      see all

    画像

    • All lanes : Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y] (ab76405) at 1/2000 dilution

      Lane 1 : HeLa cell lysates untreated.
      Lane 2 : HeLa cell lysates treated with pervanadate.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 18 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)

    参考文献

    ab76405 has not yet been referenced specifically in any publications.

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