Anti-Caveolin-1 (phospho Y14) 抗体 [EPR2288Y] (ab75876)

製品の概要

  • 製品名Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y]
    Caveolin-1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2288Y] to Caveolin-1 (phospho Y14)
  • 特異性ab75876 detects Caveolin-1 phosphorylated at tyrosine 14.
  • アプリケーション適用あり: ICC/IF, WB, ICCmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caveolin-1.
    Database link: Q03135

  • ポジティブ・コントロール
    • HeLa cell lysate treated with pervanadate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
WB 1/2000 - 1/5000. Predicted molecular weight: 20 kDa.
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    • 機能May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
    • 組織特異性Expressed in muscle and lung, less so in liver, brain and kidney.
    • 関連疾患Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
    • 配列類似性Belongs to the caveolin family.
    • 翻訳後修飾The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
    • 細胞内局在Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BSCL3 antibody
      • CAV antibody
      • CAV1 antibody
      • CAV1_HUMAN antibody
      • caveolae protein, 22 kD antibody
      • caveolin 1 alpha isoform antibody
      • caveolin 1 beta isoform antibody
      • Caveolin 1 caveolae protein 22kDa antibody
      • Caveolin-1 antibody
      • Caveolin1 antibody
      • cell growth-inhibiting protein 32 antibody
      • CGL3 antibody
      • LCCNS antibody
      • MSTP085 antibody
      • OTTHUMP00000025031 antibody
      • PPH3 antibody
      • VIP 21 antibody
      • VIP21 antibody
      see all

    Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] 画像

    • All lanes : Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876) at 1/2000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 20 kDa
      Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    • Dot blot analysis of Caveolin-1 (phospho Y14) phospho peptide (Lane 1), Caveolin-1 Non-phospho peptide (Lane 2) labelling Caveolin-1 (phospho Y14) with ab75876 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and diluting buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876) 使用論文

    ab75876 has not yet been referenced specifically in any publications.

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