Anti-Caveolin-1 抗体 [E249] - Caveolae Marker (ab32577)


  • 製品名Anti-Caveolin-1 antibody [E249] - Caveolae Marker
    Caveolin-1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E249] to Caveolin-1 - Caveolae Marker
  • 特異性This antibody should recognize both alpha and beta form of Caveolin-1.
  • アプリケーション適用あり: ICC/IF, IHC-P, ICC, WBmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caveolin-1 aa 150-250.
    Database link: Q03135

  • ポジティブ・コントロール
    • A431 cell lysate, human urinary bladder tissue,human lung tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Alternative versions available:

    Anti-Caveolin-1 antibody (Alexa Fluor® 488) [E249] - Caveolae Marker (ab185043)

    Anti-Caveolin-1 antibody (Alexa Fluor® 647) [E249] - Caveolae Marker (ab193892)

    Anti-Caveolin-1 antibody (HRP) [E249] - Caveolae Marker (ab193893)



Our Abpromise guarantee covers the use of ab32577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 19808975
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 0.01M Sodium Citrate Buffer, pH 6.0.
ICC 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
    • 組織特異性Expressed in muscle and lung, less so in liver, brain and kidney.
    • 関連疾患Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
    • 配列類似性Belongs to the caveolin family.
    • 翻訳後修飾The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
    • 細胞内局在Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BSCL3 antibody
      • CAV antibody
      • CAV1 antibody
      • CAV1_HUMAN antibody
      • caveolae protein, 22 kD antibody
      • caveolin 1 alpha isoform antibody
      • caveolin 1 beta isoform antibody
      • Caveolin 1 caveolae protein 22kDa antibody
      • Caveolin-1 antibody
      • Caveolin1 antibody
      • cell growth-inhibiting protein 32 antibody
      • CGL3 antibody
      • LCCNS antibody
      • MSTP085 antibody
      • OTTHUMP00000025031 antibody
      • PPH3 antibody
      • VIP 21 antibody
      • VIP21 antibody
      see all

    Anti-Caveolin-1 antibody [E249] - Caveolae Marker 画像

    • Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577) + A431 cell lysate

      Predicted band size : 20 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human urinary bladder tissue, staining Caveolin-1 with ab32577 at 1/250 dilution.

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung tissue, staining Caveolin-1 with ab32577 at 1/250 µg/ml.

    • Immunohistochemical analysis of developing Human lung tissue, staining Caveolin-1 with ab32577 at 1/250 dilution.

    Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577) 使用論文

    This product has been referenced in:
    • Qayyum T  et al. The interrelationships between Src, Cav-1 and RhoGD12 in transitional cell carcinoma of the bladder. Br J Cancer 106:1187-95 (2012). Read more (PubMed: 22353809) »
    • Kaarteenaho R  et al. Epithelial N-cadherin and nuclear ß-catenin are up-regulated during early development of human lung. BMC Dev Biol 10:113 (2010). IHC-P ; Human . Read more (PubMed: 21080917) »

    See all 4 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Kidney)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
    Permeabilization Yes - Wash Buffer from Dako with Tween
    Specification Kidney
    Fixative Paraformaldehyde

    Mr. Rudolf Jung

    Verified customer

    投稿 Jun 17 2016

    Thank you for contacting us.

    Regarding antibodies ab79397 and ab32577, they both have been tested in IHC with paraffin embedded sections in the species stated on the datasheet. We don’t have preliminary data using them with frozen secti...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HUVEC)
    Loading amount 100000 cells
    Specification HUVEC
    Treatment various doses of VEGF for 24h
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    投稿 May 05 2008