製品の概要

  • 製品名Anti-Caveolin-1 antibody
    Caveolin-1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Caveolin-1
  • 特異性Detects caveolin-1.
  • アプリケーション適用あり: ICC/IF, IHC-Fr, IP, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Goat, Hamster, Dog, Human
    交差が予測される動物種: Sheep, Rabbit, Horse, Cow, Cat, Pig, Chimpanzee, Gorilla, African Green Monkey, Loxodonta Africana (African bush elephant)
  • 免疫原

    Synthetic peptide corresponding to Human Caveolin-1 aa 1-17.
    Sequence:

    MSGGKYVDSEGHLYTVP


    Database link: Q03135
    (Peptide available as ab4928)

  • ポジティブ・コントロール
    • Human lung, heart or spleen
  • 特記事項

    This antibody can be used as a marker for lipid raft fractions.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500. PubMed: 17502389
IHC-Fr Use at an assay dependent concentration. PubMed: 20351069
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Caveolin-1 peptide (ab4928).
IHC-P Use at an assay dependent concentration.

ターゲット情報

Anti-Caveolin-1 antibody 画像

  • All lanes : Anti-Caveolin-1 antibody (ab2910) at 1.5 µg/ml

    Lane 1 : Human lung
    Lane 2 : Human heart
    Lane 3 : Human spleen

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa Fluor anti-rabbit at 1/5000 dilution

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded human cardiac tissue, labelling Caveolin 1 with ab2910 at a dilution of 1/10000 incubated for 2 hours at 21°C in TBS / BSA / Aside solution. Heat mediated antigen retrival was performed with citric acid. Blocking was with 1% BSA incubated for 10 minutes at 21°C. Secondary used was a goat anti-rabbit polyclonal biotin conjugate at 1/300.Image shows strong immunopositivity at the membranes of cardiomyocytes ( invaginations of immunostaining show points of branching of each myocyte). There is intense positivity in the smooth muscle of of arterioles. Not seen is the intense positivity of what seems to be the endothelial lining cells of the endocardium.

    See Abreview

  • Rat astrocytes stained with fluorescently labelled Caveolin-1 antibody. Primary antibody is ab2910 (dilution 1/500) and the secondary antibody is Texas red labelled anti-rabbit IgG (dilution1/1000).

    This image was kindly supplied as part of the review submitted by Donghui Zhu.

  • ab2910 at 1/250 dilution staining mouse NIH 3T3 cells by Immunocytochemistry (panel B). The antibody was incubated with the paraformaldehyde fixed cells for 12 hours. Bound antibody was detected using an Alexa Fluor ® 594 conjugated Goat anti-rabbit antibody (ab150080). Panel A shows staining with a mouse anti-Caveolin 1 antibody (clone 2297). Panel C shows the merged image.

    This image is courtesy of an Abreview by William Ackerman.

    See Abreview

  • ab2910 staining Caveolin-1 - Caveolae Marker in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/200 in PBS + 0.05% Saponin) for 1 hour at 37°C. A Cy3®-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab2910 diluted 1/500 and was incubated with A549 whole cell lysate and a Protein A/G matrix for 16 hours at 4°C to achieve immunoprecipitation.  400 µg of lysate was present in the input.

    An HRP-conjugated goat anti-rabbit was used for the Western Blot step.

    Lane 1: Whole Cell

    Lane 2: IP-cav-1

    Lane 3: Unrelated antibody

    Lane 4: Unrelated antibody

    See Abreview



  • Predicted band size : 20 kDa

    Western blot of caveolin-1 on rat heart protein extract using ab2910.

Anti-Caveolin-1 antibody (ab2910) 使用論文

This product has been referenced in:
  • Bourseau-Guilmain E  et al. Hypoxia regulates global membrane protein endocytosis through caveolin-1 in cancer cells. Nat Commun 7:11371 (2016). ICC/IF . Read more (PubMed: 27094744) »
  • Rodriguez-Walker M  et al. Role of plasma-membrane-bound sialidase NEU3 in clathrin-mediated endocytosis. Biochem J 470:131-44 (2015). Read more (PubMed: 26251452) »

See all 34 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Goat Tissue sections (Myocardium)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Myocardium
Blocking step (agent) for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 28 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (neuron)
Permeabilization Yes - 0.2%Triton
Specification neuron
Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 13 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (COS-7)
Permeabilization Yes - 0.2%Triton
Specification COS-7
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative Formaldehyde
Username

Ms. YA ZHOU

Verified customer

投稿 Oct 06 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cow Tissue sections (Heart muscle)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Heart muscle
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Heart muscle)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Heart muscle
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Heart muscle)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Heart muscle
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (Heart muscle)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Heart muscle
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Heart)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Heart
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Heart muscle)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Heart muscle
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Heart)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Heart
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 21 2015

1-10 of 58 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"