Anti-CaV1.3 抗体 [S48A-9] (ab85491)

製品の概要

  • 製品名Anti-CaV1.3 antibody [S48A-9]
    CaV1.3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [S48A-9] to CaV1.3
  • 特異性No cross reactivity against CaV1.2.
  • アプリケーション適用あり: IP, IHC-P, WB, ICC, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Hamster
  • 免疫原

    Synthetic peptide:

    DNKVTIDDYQEEAEDKD

    , corresponding to N terminal amino acids 859-875 of rat CaV1.3 (SwissProt P27732).

  • ポジティブ・コントロール
    • Rat brain lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
IHC-P 1/100.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 245 kDa.
ICC Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
  • 組織特異性Expressed in pancreatic islets and in brain, where it has been seen in cerebral cortex, hippocampus, basal ganglia, habenula and thalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in skeletal muscle.
  • 配列類似性Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1D subfamily.
  • ドメインEach of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • alpha-1 polypeptide antibody
    • CAC1D_HUMAN antibody
    • CACH3 antibody
    • CACN4 antibody
    • CACNA 1D antibody
    • Cacna1d antibody
    • CACNL1A2 antibody
    • Calcium channel antibody
    • Calcium channel L type alpha 1 polypeptide isoform 2 antibody
    • Calcium channel neuroendocrine/brain type alpha 1 subunit antibody
    • Calcium channel voltage dependent L type alpha 1 antibody
    • Calcium channel voltage dependent L type alpha 1D subunit antibody
    • Cav1.3 antibody
    • CCHL1A2 antibody
    • isoform 2 antibody
    • L type antibody
    • PASNA antibody
    • SANDD antibody
    • Voltage dependent L type calcium channel subunit alpha 1D antibody
    • Voltage gated calcium channel alpha 1 subunit antibody
    • Voltage gated calcium channel alpha subunit Cav1.3 antibody
    • Voltage gated calcium channel subunit alpha Cav1.3 antibody
    • Voltage-dependent L-type calcium channel subunit alpha-1D antibody
    • Voltage-gated calcium channel subunit alpha Cav1.3 antibody
    see all

Anti-CaV1.3 antibody [S48A-9] 画像

  • ab85491 staining CaV1.3 in mouse backskin tissue by IHC-P (Bouin's fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval using microwave in citrate buffer. The primary antibody was used at 1/100 dilution and then sections were incubated with Fluorophore conjugated goat anti mouse at 1/50 dilution.

  • ab85491 staining CaV1.3 in human differentiated iPS cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.05% Tween-20 and then blocked using 3% BSA for 1 hour at 21°C. Samples were then incubated with primary antibody at a 1/100 dilution for 12 hours at 4°C. The secondary antibody used was a anti-mouse IgG (H+L) conjugated to Alexa Fluor® 594 (pink) used at a 1/400 dilution.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab85491 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85491, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-CaV1.3 antibody [S48A-9] (ab85491) 使用論文

ab85491 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (differentiated iPS cells)
Specification differentiated iPS cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Tween-20
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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投稿 Oct 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"