Anti-Cathepsin V 抗体 (ab124656)
Key features and details
- Rabbit polyclonal to Cathepsin V
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-Cathepsin V antibody
Cathepsin V 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cathepsin V -
由来種
Rabbit -
特異性
ab124656 detects endogenous levels of fragment of activated CATL2 resulting from cleavage adjacent to Leu114. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, African green monkey -
免疫原
A synthetic peptide, corresponding to internal sequence amino acids of Human Cathepsin V.
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ポジティブ・コントロール
- Human breast tissue; 293 and COS cell extracts, treated with etoposide 25uM 1h.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS (without Mg2+, Ca2+) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124656の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 37 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 37 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Cysteine protease. May have an important role in corneal physiology. -
組織特異性
Predominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin. -
配列類似性
Belongs to the peptidase C1 family. -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 1515 Human
- Omim: 603308 Human
- SwissProt: O60911 Human
- Unigene: 610096 Human
- Unigene: 621851 Human
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別名
- CAT L2 antibody
- Cathepsin L2 antibody
- Cathepsin U antibody
see all
画像
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ab124656, at 1/500 dilution, staining Cathepsin V in Formalin-Fixed, Paraffin-Embedded Human breast tissue by Immunohistochemistry.
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All lanes : Anti-Cathepsin V antibody (ab124656) at 1/500 dilution
Lane 1 : 293 cell extracts, treated with etoposide 25uM 1h
Lane 2 : COS cell extracts, treated with etoposide 25uM 1h
Lane 3 : 293 cell extracts, treated with etoposide 25uM 1h with synthesized peptide
Predicted band size: 37 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab124656 は 2 報の論文で使用されています。
- Panwar P et al. Expression of elastolytic cathepsins in human skin and their involvement in age-dependent elastin degradation. Biochim Biophys Acta Gen Subj 1864:129544 (2020). PubMed: 32007579
- Matsukawa S et al. Possible Association between Cathepsin V and the Development of Placenta Accreta Spectrum Disorders. Gynecol Obstet Invest 84:396-406 (2019). PubMed: 30759440