製品の概要

  • 製品名
    Cathepsin S Activity Assay Kit (Fluorometric)
    Cathepsin S キット 製品一覧
  • サンプルの種類
    Tissue Extracts, Cell Lysate
  • アッセイタイプ
    Enzyme activity
  • 全工程の試験時間
    2h 00m
  • 製品の概要

    Cathepsin S Activity Assay Kit (ab65307) is a fluorescence-based assay that utilizes the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-S will cleave the synthetic substrate VVR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 ラベル 100 tests
    CS Cell Lysis Buffer WM 1 x 25ml
    CS Inhibitor (1 mM) Red 1 x 20µl
    CS Reaction Buffer NM 1 x 5ml
    CS Substrate Ac-VVR-AFC (10 mM) Brown 1 x 200µl
  • 研究分野
  • 関連性
    The cathepsin family of proteases contains several diverse classes of enzymes. The cysteine protease class comprises cathepsin B, H, K, L, O, and S. The aspartyl protease class contains cathepsin D and E. Cathepsin G belongs to the serine protease class. Cathepsins are involved in various cellular events such as peptide biosynthesis, protein degradation, and apoptosis. Cathepsin S has been shown to be able to function as an elastase over a broad pH range in alveolar macrophages.
  • 細胞内局在
    Lysosome
  • 別名
    • CathepsinS
    • CTSS
    • MGC3886

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab65307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

画像

  • ab65307 Cathepsin S Activity
    ab65307 Cathepsin S Activity

    Cathepsin S Activity measured in tissue lysates (each with protein concentration of 4 mg/mL) after 1 hour of incubation.

  • ab65307 Cathepsin S Activity
    ab65307 Cathepsin S Activity

    Cathepsin S Activity measured in cell lysates (each with cells concentration of 4e7 cells/mL) after 1 hour of incubation with and without inhibitor (I).

プロトコール

参考文献

This product has been referenced in:
  • Zhang J  et al. Palmitate impairs angiogenesis via suppression of cathepsin activity. Mol Med Rep 15:3644-3650 (2017). Read more (PubMed: 28440480) »
  • Tjondrokoesoemo A  et al. Genetic overexpression of Serpina3n attenuates muscular dystrophy in mice. Hum Mol Genet 25:1192-202 (2016). Functional Studies ; Mouse . Read more (PubMed: 26744329) »

See all 3 Publications for this product

ab65307 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

4 reviews or Q&A

10
Votes: Highest First

Inquiry: Hi there, I just wanted to ask if the fluresence-based activity kits that you have available for cathepsin B, L and S ab65307 cathepsin s for e.g. are suitable for the use with conditioned media for cell lines. I have been looking through the ...

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I can confirm that the use of conditioned medium does not change the Cathepsin S assay in any way. Typically the conditioned medium is removed and retained and the cells are lysed to assay for Cathepsin activity. The Cathepsin activity in the medium is...

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Can I prepare only one cell lysate to use with all 3 kits ab65302, ab306 and ab307?

We do not recommend to prepare one lysate to use with ab65302, ab65306 and ab65307.
It is important to prepare separate lysates for each kit, using the specific lysis buffer provided. This is because the cathepsins have very specific pH and other c...

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both kits: for use with tissue samples - any specific tips and instructions?
any substances to avoid (cathepsin can be instable at a certain pH)


The lab let me know that they do not have any additional tips for tissue processing other than the ones we mention on the FAQ site.


12. How should tissue be prepared for the kits?

Samples should be completely homogenized, pr...

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Hi, I am interested in doing cathepsin s activity. Can I use tissue lysate for this assay or do people usually use cell lysate? Does this come with a positive control?



Yes you can use cell lysates or tissue extract samples with this kit.

This kit does not come with a positive control.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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