Cathepsin S Activity Assay Kit (Fluorometric) (ab65307)
製品の概要
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製品名Cathepsin S Activity Assay Kit (Fluorometric)
Cathepsin S キット 製品一覧 -
サンプルの種類Tissue Extracts, Cell Lysate
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アッセイタイプEnzyme activity
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全工程の試験時間2h 00m
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製品の概要
Cathepsin S Activity Assay Kit (ab65307) is a fluorescence-based assay that utilizes the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-S will cleave the synthetic substrate VVR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.
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特記事項
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
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アプリケーション適用あり: Functional Studiesmore details
製品の特性
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保存方法Store at -20°C. Please refer to protocols.
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内容 ラベル 100 tests CS Cell Lysis Buffer WM 1 x 25ml CS Inhibitor (1 mM) Red 1 x 20µl CS Reaction Buffer NM 1 x 5ml CS Substrate Ac-VVR-AFC (10 mM) Brown 1 x 200µl -
研究分野
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関連性The cathepsin family of proteases contains several diverse classes of enzymes. The cysteine protease class comprises cathepsin B, H, K, L, O, and S. The aspartyl protease class contains cathepsin D and E. Cathepsin G belongs to the serine protease class. Cathepsins are involved in various cellular events such as peptide biosynthesis, protein degradation, and apoptosis. Cathepsin S has been shown to be able to function as an elastase over a broad pH range in alveolar macrophages.
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細胞内局在Lysosome
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別名
- CathepsinS
- CTSS
- MGC3886
関連製品
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アプリケーション
Our Abpromise guarantee covers the use of ab65307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies | Use at an assay dependent dilution. |
Cathepsin S Activity Assay Kit (Fluorometric) 画像
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ab65307 Cathepsin S ActivityCathepsin S Activity measured in tissue lysates (each with protein concentration of 4 mg/mL) after 1 hour of incubation.
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ab65307 Cathepsin S ActivityCathepsin S Activity measured in cell lysates (each with cells concentration of 4e7 cells/mL) after 1 hour of incubation with and without inhibitor (I).
プロトコール
Cathepsin S Activity Assay Kit (Fluorometric) (ab65307) 使用論文
This product has been referenced in:
- Tjondrokoesoemo A et al. Genetic overexpression of Serpina3n attenuates muscular dystrophy in mice. Hum Mol Genet 25:1192-202 (2016). Functional Studies ; Mouse . Read more (PubMed: 26744329) »
- Chiang L et al. Rab27b regulates exocytosis of secretory vesicles in acinar epithelial cells from the lacrimal gland. Am J Physiol Cell Physiol 301:C507-21 (2011). Read more (PubMed: 21525430) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"