All lanes : Anti-Cathepsin L antibody (ab95154) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/3000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 37 kDa Observed band size : 37 kDa Additional bands at : 25 kDa (possible cleavage fragment),35 kDa (possible cleavage fragment),71 kDa. We are unsure as to the identity of these extra bands.