製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Cathepsin L
  • 由来種
    Rabbit
  • 特異性
    Reacts specifically with Cathepsin L protease 36 kDa wild type protein of C.elegans
  • アプリケーション
    適用あり: Electron Microscopy, WB, ICC/IFmore details
  • 種交差性
    交差種: Caenorhabditis elegans
  • 免疫原

    Synthetic peptide derived from mature region of Cathepsin L (Caenorhabditis elegans)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Electron Microscopy Use at an assay dependent dilution.
WB 1/200 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ICC/IF 1/100.

ターゲット情報

  • 機能
    Important for the overall degradation of proteins in lysosomes.
  • 配列類似性
    Belongs to the peptidase C1 family.
  • 細胞内局在
    Lysosome.
  • Information by UniProt
  • 別名
    • cathepsin L, 1 b antibody
    • Cathepsin L1 antibody
    • Cathepsin L1 light chain antibody
    • CathepsinL antibody
    • CATL antibody
    • CATL1_HUMAN antibody
    • cb15 antibody
    • CTSL antibody
    • CTSL1 antibody
    • ctsl1b antibody
    • FLJ31037 antibody
    • hgg1 antibody
    • Major excreted protein antibody
    • MEP antibody
    • MGC123162 antibody
    • wu:fb30g09 antibody
    see all

画像

  • Anti-Cathepsin L antibody (ab58991) at 1/500 dilution + Whole cell lysate prepared from human HCT116 cell at 10 µg

    Secondary
    HRP-conjugated donkey ppolyclonal to rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 35 kDa (why is the actual band size different from the predicted?)


    Exposure time: 2 minutes

    See Abreview

  • ICC/IF image of ab58991 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58991, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:

See all 8 Publications for this product

レビューと Q&A

Application
IHC - Wholemount
Sample
Zebrafish Embryo (Hatching Gland)
Specification
Hatching Gland
Username

Abcam user community

Verified customer

投稿 Aug 11 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (kidney)
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Dec 11 2014

Thank you for contacting us.

The supplying lab let me know that the antibody was used in EM based on the following information:



Immuno electron microscopy (IEM)
C. elegans gravid hermaphrodites were permeabilised by...

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Thank you for your email.

I am checking this information with the lab and let you know what I find out.

Currently, we are not aware of publications that have used the antibody in EM so far.

Please do not hesitate to cont...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
10 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Apr 09 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116)
Loading amount
10 µg
Specification
HCT116
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Apr 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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