Anti-Cathepsin K 抗体 (ab85716)
Key features and details
- Rabbit polyclonal to Cathepsin K
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cathepsin K antibody
Cathepsin K 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cathepsin K -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog, Pig, Non human primates -
免疫原
Synthetic peptide corresponding to Human Cathepsin K aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab97980) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following lysates: Human Bone Marrow Tissue; Human Lung Tissue; U2OS Whole Cell; MCF7 Whole Cell; MDA-MB-231 Whole Cell; MDA-MB-361 Whole Cell; Saos 2 Whole Cell.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85716の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 37 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 37 kDa). |
ターゲット情報
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機能
Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. -
組織特異性
Predominantly expressed in osteclasts (bones). -
関連疾患
Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. -
配列類似性
Belongs to the peptidase C1 family. -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 513038 Cow
- Entrez Gene: 1513 Human
- Entrez Gene: 13038 Mouse
- Entrez Gene: 397569 Pig
- Entrez Gene: 29175 Rat
- Omim: 601105 Human
- SwissProt: Q5E968 Cow
- SwissProt: Q3ZKN1 Dog
see all -
別名
- Cathepsin K antibody
- Cathepsin O antibody
- Cathepsin O1 antibody
see all
画像
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All lanes : Anti-Cathepsin K antibody (ab85716) at 1 µg/ml
Lane 1 : Bone marrow (Human) Tissue Lysate
Lane 2 : Lung (Human) Tissue Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
We hypothesize that the 24 kDa band represents the mature form of Cathepsin K.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab85716 は 1 報の論文で使用されています。
- Maatouk L et al. TLR9 activation via microglial glucocorticoid receptors contributes to degeneration of midbrain dopamine neurons. Nat Commun 9:2450 (2018). PubMed: 29934589