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Cathepsin H Activity Assay Kit (Fluorometric) (ab65305) is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate Arginine labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate R-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. The Cathepsin H assay is simple, straightforward, and can be adapted to 96-well plate assays. Assay conditions have been optimized to obtain the maximal activity.
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Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
|CH Cell Lysis Buffer||WM||1 x 25ml|
|CH Inhibitor (1 mM)||Red||1 x 20µl|
|CH Reaction Buffer||NM||1 x 5ml|
|CH Substrate R-AFC (10 mM)||Amber||1 x 200µl|
Our Abpromise guarantee covers the use of ab65305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
ab65305 has not yet been referenced specifically in any publications.