Cathepsin G Inhibitor Screening Assay Kit (Colorimetric) (ab204693)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Inhibitor compounds
製品の概要
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製品名
Cathepsin G Inhibitor Screening Assay Kit (Colorimetric)
Cathepsin G キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Inhibitor compounds -
アッセイタイプ
Enzyme activity -
製品の概要
Cathepsin G Inhibitor Screening Assay Kit (Colorimetric) (ab204693) uses the ability of active Cathepsin G to cleave a synthetic pNA (4-Nitroaniline)-based peptide substrate to release pNA, which can be easily quantified using a colorimetric microplate reader. In the presence of a Cathepsin G inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the pNA absorbance. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin G.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K162 Cathepsin G Inhibitor Screening Kit (Colorimetric). K162-100 is the same size as the 100 test size of ab204693.
Cathepsin G (CTSG, EC 3.4.21.20) is a Serine protease found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Cathepsin G Inhibitor 1 x 20µl Cathepsin G Substrate 1 x 200µl CTSG Assay Buffer 1 x 20ml Human Cathepsin G II 1 vial -
研究分野
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機能
Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
細胞内局在
Cell surface. - Information by UniProt
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別名
- CATG
- CATG_HUMAN
- Cathepsin G
see all
関連製品
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Related Products
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab204693 は 1 報の論文で使用されています。
- Goldberg EL et al. ß-Hydroxybutyrate Deactivates Neutrophil NLRP3 Inflammasome to Relieve Gout Flares. Cell Rep 18:2077-2087 (2017). PubMed: 28249154