製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Cathepsin F
  • 由来種
    Goat
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Dog
  • 免疫原

    Synthetic peptide:

    CGVNTMASSAVVD

    , corresponding to C terminal amino acids 472-484 of Human Cathepsin F

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab36161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    Peptide ELISA: Antibody detection limit dilution 1:32,000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 53 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
    • 組織特異性
      High expression levels in heart, skeletal muscle, brain, testis and ovary; moderate levels in prostate, placenta, liver and colon; and no detectable expression in peripheral leukocytes and thymus.
    • 配列類似性
      Belongs to the peptidase C1 family.
    • 細胞内局在
      Lysosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AI481912 antibody
      • CATF_HUMAN antibody
      • Cathepsin F antibody
      • CathepsinF antibody
      • CATSF antibody
      • CLN13 antibody
      • Ctsf antibody
      • EC 3.4.22.41 antibody
      see all

    画像

    • Anti-Cathepsin F antibody (ab36161) at 0.3 µg/ml + Human Ovary lysate (35µg protein in RIPA buffer).

      Predicted band size: 53 kDa



      Primary incubation was 1 hour. Detected by chemiluminescence.

      Experiments gave bands at approx 30kDa and 35kDa in Human Prostate and Ovary lysates after 0.3µg/ml antibody staining. These bands correspond to earlier findings in literature (Wang et al, J Biol Chem. 1998 Nov 27;273(48):32000-8; PMID: 9822672). This protein has a calculated MW of 53.4kDa according to NP_003784.2.

    プロトコール

    参考文献

    This product has been referenced in:
    • Jeric B  et al. N-terminally truncated forms of human cathepsin F accumulate in aggresome-like inclusions. Biochim Biophys Acta 1833:2254-66 (2013). Human . Read more (PubMed: 23684953) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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