製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Cathepsin E
  • 特異性
    This antibody is specific for Cathepsin E.
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide made to the C-terminus of mouse Cathepsin E.

  • ポジティブ・コントロール
    • Murine reticulocyte lysate. Cathepsin E is expressed abundantly in the stomach, Clara cells and alveolar macrophages of the lung, brain microglia, spleen, and activated B lymphocytes. It is not expressed in resting B lymphocytes.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab36996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 42 kDa.
ICC/IF 1/200.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.
  • 組織特異性
    Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B-lymphocytes.
  • 配列類似性
    Belongs to the peptidase A1 family.
  • 翻訳後修飾
    Glycosylated. The nature of the carbohydrate chain varies between cell types. In fibroblasts, the proenzyme contains a high mannose-type oligosaccharide, while the mature enzyme contains a complex-type oligosaccharide. In erythrocyte membranes, both the proenzyme and mature enzyme contain a complex-type oligosaccharide.
    Two forms are produced by autocatalytic cleavage, form I begins at Ile-54, form II begins at Thr-57.
  • 細胞内局在
    Endosome. The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CATE antibody
    • CATE_HUMAN antibody
    • Cathepsin E antibody
    • Cathepsin E form II antibody
    • CTSE antibody
    • Erythrocyte membrane aspartic proteinase antibody
    • Slow moving proteinase antibody
    see all

画像

  • Anti-Cathepsin E antibody (ab36996) at 1/1000 dilution + Murine reticulocyte lysate at 10 µg

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ab36996 staining Cathepsin E at a dilution of 1/2000 in Human stomach and tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The sections were PFA-fixed and subjected to Heat-mediated antigen retrieval in TRIS EDTA Buffer pH 9.0 and permeabilization with DAKO wash buffer with Tween. The primary antibody was diluted 1/2000 in DAKO blocking dilution buffer and incubated with the sample for 30 minutes at 20°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Fisher OM  et al. High Expression of Cathepsin E in Tissues but Not Blood of Patients with Barrett's Esophagus and Adenocarcinoma. Ann Surg Oncol 22:2431-8 (2015). IHC . Read more (PubMed: 25348778) »
  • Fristrup N  et al. Cathepsin E, maspin, Plk1, and survivin are promising prognostic protein markers for progression in non-muscle invasive bladder cancer. Am J Pathol 180:1824-34 (2012). IHC-P ; Human . Read more (PubMed: 22449953) »

See all 3 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Stomach and Tonsil)
Specification
Stomach and Tonsil
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9.0
Permeabilization
Yes - Wash Buffer from DAKO with tween
Username

Mr. Rudolf Jung

Verified customer

投稿 May 04 2011

Application
Western blot
Sample
Mouse Tissue lysate - whole (Pancreas)
Loading amount
20 µg
Specification
Pancreas
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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