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RabMAb

Anti-Cathepsin D 抗体 [EPR3056Y] (ab75811)

製品の概要

  • 製品名
    Anti-Cathepsin D antibody [EPR3056Y]
    Cathepsin D 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3056Y] to Cathepsin D
  • アプリケーション
    適用あり: Flow Cyt, WB, IHC-P, ICCmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human Cathepsin D

  • ポジティブ・コントロール
    • MCF-7 and SKBR3 cell lysates. Human liver tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
WB
IHC-P
ICC
  • 追加情報
    ICC: 1/100 - 1/250.
    IHC-P: 1/500. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/1000 - 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
    • 組織特異性
      Expressed in the aorta extrcellular space (at protein level).
    • 関連疾患
      Ceroid lipofuscinosis, neuronal, 10
    • 配列類似性
      Belongs to the peptidase A1 family.
      Contains 1 peptidase A1 domain.
    • 翻訳後修飾
      N- and O-glycosylated.
    • 細胞内局在
      Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
    • Information by UniProt
    • 参照データベース
    • 別名
      • CatD antibody
      • CATD_HUMAN antibody
      • Cathepsin D antibody
      • Cathepsin D heavy chain antibody
      • CD antibody
      • Ceroid lipofuscinosis neuronal 10 antibody
      • CLN10 antibody
      • CPSD antibody
      • ctsd antibody
      • Epididymis secretory sperm binding protein Li 130P antibody
      • HEL S 130P antibody
      • Lysosomal aspartyl peptidase antibody
      • Lysosomal aspartyl protease antibody
      • MGC2311 antibody
      see all

    画像

    • All lanes : Anti-Cathepsin D antibody [EPR3056Y] (ab75811) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : SKBR3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 46 kDa
      Observed band size : 15,46 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human liver using ab75811 at 1/500 dilution.

    参考文献

    ab75811 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (small intestine, ganglion cells)
    Specification
    small intestine, ganglion cells
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate, 100C 20 minutes
    Permeabilization
    No
    Username

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    投稿 Oct 22 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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