Anti-Cathepsin D 抗体 [EPR3056Y] (ab75811)

製品の概要

  • 製品名Anti-Cathepsin D antibody [EPR3056Y]
    Cathepsin D 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3056Y] to Cathepsin D
  • アプリケーション適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human Cathepsin D

  • ポジティブ・コントロール
    • MCF-7 and SKBR3 cell lysates. Human liver tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab75811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
ICC
  • 追加情報ICC: 1/100 - 1/250.
    IHC-P: 1/500. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/1000 - 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
    • 組織特異性Expressed in the aorta extrcellular space (at protein level).
    • 関連疾患Ceroid lipofuscinosis, neuronal, 10
    • 配列類似性Belongs to the peptidase A1 family.
      Contains 1 peptidase A1 domain.
    • 翻訳後修飾N- and O-glycosylated.
    • 細胞内局在Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
    • Information by UniProt
    • 参照データベース
    • 別名
      • CatD antibody
      • CATD_HUMAN antibody
      • Cathepsin D heavy chain antibody
      • CD antibody
      • Ceroid lipofuscinosis neuronal 10 antibody
      • CLN10 antibody
      • CPSD antibody
      • ctsd antibody
      • Epididymis secretory sperm binding protein Li 130P antibody
      • HEL S 130P antibody
      • Lysosomal aspartyl peptidase antibody
      • Lysosomal aspartyl protease antibody
      • MGC2311 antibody
      see all

    Anti-Cathepsin D antibody [EPR3056Y] 画像

    • All lanes : Anti-Cathepsin D antibody [EPR3056Y] (ab75811) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : SKBR3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 46 kDa
      Observed band size : 15,46 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human liver using ab75811 at 1/500 dilution.

    Anti-Cathepsin D antibody [EPR3056Y] (ab75811) 使用論文

    ab75811 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (small intestine, ganglion cells)
    Specification small intestine, ganglion cells
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, 100C 20 minutes
    Permeabilization No
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    投稿 Oct 22 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"