製品の概要

  • 製品名
    Anti-Cathepsin D antibody [CTD-19]
    Cathepsin D 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CTD-19] to Cathepsin D
  • 特異性
    Recognizes cathepsin D (34 kDa and weaker band at 52 kDa) in immunoblotting. Does not react with bovine cathepsin D and B, or with human cathepsin types B, C, G or H.
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Full length native protein (purified) (Human liver).

  • ポジティブ・コントロール
    • Human breast carcinoma tissue or cell line extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 52 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/200.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 24833013

ターゲット情報

  • 機能
    Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • 組織特異性
    Expressed in the aorta extrcellular space (at protein level).
  • 関連疾患
    Ceroid lipofuscinosis, neuronal, 10
  • 配列類似性
    Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • 翻訳後修飾
    N- and O-glycosylated.
  • 細胞内局在
    Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt
  • 参照データベース
  • 別名
    • CatD antibody
    • CATD_HUMAN antibody
    • Cathepsin D antibody
    • Cathepsin D heavy chain antibody
    • CD antibody
    • Ceroid lipofuscinosis neuronal 10 antibody
    • CLN10 antibody
    • CPSD antibody
    • ctsd antibody
    • Epididymis secretory sperm binding protein Li 130P antibody
    • HEL S 130P antibody
    • Lysosomal aspartyl peptidase antibody
    • Lysosomal aspartyl protease antibody
    • MGC2311 antibody
    see all

Anti-Cathepsin D antibody [CTD-19] 画像

  • Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution + MDA MB 231 whole cell lysate at 20 µg

    Secondary
    HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 28,48,50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 22°C.

    Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.

    See Abreview

  • ab6313 staining Cathepsin D in DU145 human prostate cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 and blocked with 5% BSA at room temperature for 20 minutes. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour. A CF488A-conjugated goat anti-mouse IgG (H+L) polyclonal was used as the secondary antibody (1/500).

    See Abreview

  • ab6313 staining Cathepsin D in HepaRG cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 in PBS and blocked with 1% milk at room temperature for 20 minutes. Samples were incubated with primary antibody (1µg/ml in 1% milk) for 30 minutes. An undiluted Alexa Fluor® 594-conjugated goat anti-mouse IgG  polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cells

Anti-Cathepsin D antibody [CTD-19] (ab6313) 使用論文

This product has been referenced in:
  • Valdez HA  et al. Bordetella pertussis modulates human macrophage defense gene expression. Pathog Dis 74:N/A (2016). Read more (PubMed: 27465637) »
  • Tokuda E  et al. Low autophagy capacity implicated in motor system vulnerability to mutant superoxide dismutase. Acta Neuropathol Commun 4:6 (2016). WB ; Human . Read more (PubMed: 26810478) »

See all 17 Publications for this product

Product Wall

Application
Western blot
Sample
Human Tissue lysate - whole (CSF)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
CSF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 11 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (brain tissue)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 11 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (10)
Sample
Human Cell lysate - other (Glioblastoma cell lines)
Specification
Glioblastoma cell lines
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Mr. Achilleas Mitrakas

Verified customer

投稿 Apr 25 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (DU145 Human prostate)
Specification
DU145 Human prostate
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

投稿 Nov 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (adipocyte tissue)
Loading amount
35 µg
Specification
adipocyte tissue
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 18 2012

Vielen Dank für Ihre Anfrage die Herr Hryca uns in den technischen Dienst weitergeleitet hat.

Ich habe geschaut, und wir haben viele möglichen positiven Kontrollen für Sie. Können Sie mir bitte noch sagen, welche Art von p...

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I have looked at the protocols used and everything seems fine to me. Therefore, I am more than happy to arrange for one vial of ab21679 Anti-Clathrin heavy chain antibody as a free of charg...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepaRG)
Specification
HepaRG
Fixative
Formaldehyde
Permeabilization
Yes - Triton X-100 in PBS
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mrs. Alina Macovei

Verified customer

投稿 May 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepaRG)
Loading amount
10 µg
Specification
HepaRG
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Alina Macovei

Verified customer

投稿 May 03 2012

1-10 of 23 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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