製品の概要

  • 製品名Anti-Cathepsin D antibody
    Cathepsin D 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cathepsin D
  • 特異性This antibody reacts with human liver cathepsin D, and does not react with cathepsins B, H and L.
  • アプリケーション適用あり: ELISA, WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Mature form Cathepsin D.

  • ポジティブ・コントロール
    • Tested with human liver cells. IF/ICC: HeLa cell line. This antibody gave a positive result in IHC in the following FFPE tissue: Human Lung.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/4000. Predicted molecular weight: 46 kDa.
ICC/IF 1/1000.
IHC-P 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • 組織特異性Expressed in the aorta extrcellular space (at protein level).
  • 関連疾患Ceroid lipofuscinosis, neuronal, 10
  • 配列類似性Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • 翻訳後修飾N- and O-glycosylated.
  • 細胞内局在Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt
  • 参照データベース
  • 別名
    • CatD antibody
    • CATD_HUMAN antibody
    • Cathepsin D heavy chain antibody
    • CD antibody
    • Ceroid lipofuscinosis neuronal 10 antibody
    • CLN10 antibody
    • CPSD antibody
    • ctsd antibody
    • Epididymis secretory sperm binding protein Li 130P antibody
    • HEL S 130P antibody
    • Lysosomal aspartyl peptidase antibody
    • Lysosomal aspartyl protease antibody
    • MGC2311 antibody
    see all

Anti-Cathepsin D antibody 画像

  • IHC image of Cathepsin D staining in Human Lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19555, 1/2000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab19555 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19555, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Anti-Cathepsin D antibody (ab19555) 使用論文

This product has been referenced in:
  • Wallin H  et al. Cystatin C properties crucial for uptake and inhibition of intracellular target enzymes. J Biol Chem 288:17019-29 (2013). ICC/IF ; Human . Read more (PubMed: 23629651) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Colon)
Loading amount 30 µg
Specification Colon
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Mr. Rod Nibbe

Verified customer

投稿 Sep 20 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"