Anti-Cathepsin B 抗体 [EPR4323] (ab125067)


  • 製品名Anti-Cathepsin B antibody [EPR4323]
    Cathepsin B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4323] to Cathepsin B
  • アプリケーション適用あり: WB, IP, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cathepsin B aa 1-200. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • Human kidney and papillary carcinoma tissues; HeLa cells; HT1080, A375, SW480 and A549 cell line lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab125067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
    • 配列類似性Belongs to the peptidase C1 family.
    • 細胞内局在Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Amyloid precursor protein secretase antibody
      • APP secretase antibody
      • APPS antibody
      • CATB_HUMAN antibody
      • Cathepsin B heavy chain antibody
      • Cathepsin B1 antibody
      • CathepsinB antibody
      • CPSB antibody
      • CTSB antibody
      • cysteine protease antibody
      • OTTHUMP00000116009 antibody
      • OTTHUMP00000229510 antibody
      • OTTHUMP00000229511 antibody
      • OTTHUMP00000229512 antibody
      • OTTHUMP00000229514 antibody
      • OTTHUMP00000229515 antibody
      • OTTHUMP00000229516 antibody
      • Preprocathepsin B antibody
      see all

    Anti-Cathepsin B antibody [EPR4323] 画像

    • ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human kidney tissue by immunohistochemistry.
    • ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue.
    • All lanes : Anti-Cathepsin B antibody [EPR4323] (ab125067) at 1/1000 dilution

      Lane 1 : HT1080 cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Goat anti-Rabbit HRP at 1/2000 dilution
      developed using the ECL technique

      Predicted band size : 38 kDa
    • ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human papillary carcinoma tissue by immunohistochemistry.

    Anti-Cathepsin B antibody [EPR4323] (ab125067) 使用論文

    ab125067 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us and your interest in our products.
    Please find the ICC protocol used with the Anti-Cathepsin B antibody [EPR4323] (ab125067) attached to this email.
    The secondary antibody used in the staining was an Alexa Fluor 56...

    Read More