製品の概要

  • 製品名Anti-Cathepsin B antibody [CA10]
    Cathepsin B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CA10] to Cathepsin B
  • 特異性This antibody recognizes an epitope on the heavy chain of mature Cathepsin B and therefore detects both mature (~31 kDa) and pro (~43 kDa)Cathepsin B.
  • アプリケーション適用あり: IHC-P, Flow Cyt, WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Cow, Human, Pig
    非交差種: Rat
  • 免疫原

    Cathepsin B purified from human liver

  • エピトープWithin the heavy chain of mature cathepsin B
  • ポジティブ・コントロール
    • HepG2 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 38 kDa.
IP Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • 配列類似性Belongs to the peptidase C1 family.
  • 細胞内局在Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Amyloid precursor protein secretase antibody
    • APP secretase antibody
    • APPS antibody
    • CATB_HUMAN antibody
    • Cathepsin B heavy chain antibody
    • Cathepsin B1 antibody
    • CathepsinB antibody
    • CPSB antibody
    • CTSB antibody
    • cysteine protease antibody
    • OTTHUMP00000116009 antibody
    • OTTHUMP00000229510 antibody
    • OTTHUMP00000229511 antibody
    • OTTHUMP00000229512 antibody
    • OTTHUMP00000229514 antibody
    • OTTHUMP00000229515 antibody
    • OTTHUMP00000229516 antibody
    • Preprocathepsin B antibody
    see all

Anti-Cathepsin B antibody [CA10] 画像

  • ab58802 staining Cathepsin B in murine mammary gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked and then incubated with ab58802 at a 1/200 dilution for 30 minutes at 22°C. The secondary used was a biotin conjugated rabbit anti-mouse monoclonal at a 1/250 dilution.

    See Abreview

  • Anti-Cathepsin B antibody [CA10] (ab58802) at 0.25 µg/ml + Natural human Cathepsin B protein (ab90387) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • Overlay histogram showing HepG2 cells stained with ab58802 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58802, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunofluorescence analysis of U87MG cells incubated with netrin-1, staining Cathepsin B with ab58802.

Anti-Cathepsin B antibody [CA10] (ab58802) 使用論文

This product has been referenced in:
  • Hibbert B  et al. Glycogen synthase kinase-3ß inhibition augments diabetic endothelial progenitor cell abundance and functionality via cathepsin B: a novel therapeutic opportunity for arterial repair. Diabetes 63:1410-21 (2014). WB ; Human . Read more (PubMed: 24296714) »
  • Shimizu A  et al. Netrin-1 Promotes Glioblastoma Cell Invasiveness and Angiogenesis by Multiple Pathways Including Activation of RhoA, Cathepsin B, and cAMP-response Element-binding Protein. J Biol Chem 288:2210-22 (2013). ICC/IF ; Human . Read more (PubMed: 23195957) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (gall bladder cancer cell line OCUG-1)
Gel Running Conditions Reduced Denaturing (12% SDS PAGE)
Loading amount 50 µg
Specification gall bladder cancer cell line OCUG-1
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Mr. Siddharth Mehra

Verified customer

投稿 Jun 01 2016

The immunogen used to create ab58802 is purified cathepsin B from human liver. The epitope has been mapped to the heavy chain of mature cathepsin B, but no more specifics are known.

We have no data showing whether or not this antibody will cro...

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Thank you for your updates.

I am very sorry to hear that the customer is having problem with this antibody as well. It could well be that the product has been damaged during shipment to India. Currently, we have a few alternative antibodies f...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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Unfortunately, alignment of our immunogen with mosquito Cathepsin B shows identity below threshold for reactivity of this antibody in this species. An immunogen should have 85% identity with the target of inter...

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I am very pleased to hear you would like to test ab5076, ab8049, ab18258, ab58802, ab64581, ab82259 and send us your results. The testing discount doesn't apply to ab4674 since this has already been tested in Rhesus monkey and IHC so we would guarantee...

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I would like to offer some suggestions to help optimize the results:

1. First thing first: ab77355 is a goat antibody. The secondary antibody will cross reacts with Bovine Ig present in...

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I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in o...

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Thank you for taking time to check these additional details. The details provided will enable us to investigate this case. Itam very sorrythatthe results with this antibodyhave not been successful.

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

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