製品の概要
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製品名Anti-Cathepsin B antibody
Cathepsin B 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Cathepsin B
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アプリケーション適用あり: ICC/IF, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Orangutan
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免疫原
Synthetic peptide derived from within residues 200 - 300 of Human Cathepsin B.
Synthetic peptide of Human Cathepsin B.
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ポジティブ･コントロール
- This antibody gave a positive signal in the following lysates: Human Liver Tissue; Human Kidney Tissue; Human Brain Tissue; Human Placenta Tissue; HepG2 Whole Cell; HEK293 Whole Cell Lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab92955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 30, 25 kDa (predicted molecular weight: 38 kDa). |
ターゲット情報
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機能Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
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配列類似性Belongs to the peptidase C1 family.
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細胞内局在Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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参照データベース
- Entrez Gene: 1508 Human
- Omim: 116810 Human
- SwissProt: P07858 Human
- Unigene: 520898 Human
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別名
- Amyloid precursor protein secretase antibody
- APP secretase antibody
- APPS antibody
see all
Anti-Cathepsin B antibody 画像
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Western blot - Cathepsin B antibody (ab92955)All lanes : Anti-Cathepsin B antibody (ab92955) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lane 4 : Human placenta tissue lysate - total protein (ab29745)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 38 kDa
Observed band size : 25,30 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds -
Immunocytochemistry/ Immunofluorescence - Cathepsin B antibody (ab92955)ICC/IF image of ab92955 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92955 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Cathepsin B antibody (ab92955) 使用論文
ab92955 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"