Anti-Cathelicidin/CLP 抗体 (ab69484)
Key features and details
- Rabbit polyclonal to Cathelicidin/CLP
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cathelicidin/CLP antibody
Cathelicidin/CLP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cathelicidin/CLP -
由来種
Rabbit -
特異性
There is 71% homology between the immunogen and mouse cathelicidin/CLP. This antibody has been published (see PubMed: 21724991) with mouse samples, but we were unable to see an appropriately sized band in mouse spleen or small intestine by WB. It may be that certain lots have worked with mouse, but we are unable to guarantee cross-reactivity with mouse after our testing.
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アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Cathelicidin/CLP aa 50-100 (internal sequence). The exact sequence is proprietary.
Database link: P49913 -
ポジティブ・コントロール
- Human spleen tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69484の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml.
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
ターゲット情報
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機能
Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. -
組織特異性
Expressed in bone marrow and testis and neutrophils. -
配列類似性
Belongs to the cathelicidin family. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 820 Human
- Omim: 600474 Human
- SwissProt: P49913 Human
- Unigene: 51120 Human
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別名
- 18 kDa cationic antimicrobial protein antibody
- Antibacterial peptide LL-37 antibody
- Antibacterial protein FALL-39 antibody
see all
画像
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ab69484 at 5 µg/ml staining Cathelicidin/CLP in human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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Anti-Cathelicidin/CLP antibody (ab69484) at 1 µg/ml + Human spleen tissue lysate at 15 µg
Predicted band size: 19 kDa
Observed band size: 19 kDa -
Immunofluorescence of Cathelicidin/CLP in Human Spleen cells using ab69484 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab69484 は 9 報の論文で使用されています。
- Smith KJ et al. The antimicrobial peptide cathelicidin drives development of experimental autoimmune encephalomyelitis in mice by affecting Th17 differentiation. PLoS Biol 20:e3001554 (2022). PubMed: 36026478
- Porter RJ et al. Colonic epithelial cathelicidin (LL-37) expression intensity is associated with progression of colorectal cancer and presence of CD8+ T cell infiltrate. J Pathol Clin Res 7:495-506 (2021). PubMed: 33988317
- Chen X et al. Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis. Mol Med Rep 22:5243-5250 (2020). PubMed: 33174023
- Cao Y et al. LL-37 promotes neutrophil extracellular trap formation in chronic rhinosinusitis with nasal polyps. Clin Exp Allergy 49:990-999 (2019). PubMed: 31046155
- Chen S et al. Eluted 25-hydroxyvitamin D3 from radially aligned nanofiber scaffolds enhances cathelicidin production while reducing inflammatory response in human immune system-engrafted mice. Acta Biomater 97:187-199 (2019). PubMed: 31386930
- Lazzaretto B & Fadeel B Intra- and Extracellular Degradation of Neutrophil Extracellular Traps by Macrophages and Dendritic Cells. J Immunol 203:2276-2290 (2019). PubMed: 31519860
- Harman RM et al. Antimicrobial peptides secreted by equine mesenchymal stromal cells inhibit the growth of bacteria commonly found in skin wounds. Stem Cell Res Ther 8:157 (2017). PubMed: 28676123
- Li S et al. Exposure to fermentation supernatant of Staphylococcus aureus accelerated dedifferentiation of chondrocytes and production of antimicrobial peptides. J Orthop Res N/A:N/A (2017). PubMed: 28513981
- Douda DN et al. Innate immune collectin surfactant protein d simultaneously binds both neutrophil extracellular traps and carbohydrate ligands and promotes bacterial trapping. J Immunol 187:1856-65 (2011). WB ; Mouse . PubMed: 21724991