Catalase Specific Activity Assay Kit (ab118184)

製品の概要

  • 製品名Catalase Specific Activity Assay Kit
    Catalase キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    1 8 7.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    1 4 9.9%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture extracts, Tissue Extracts
  • アッセイタイプEnzyme activity
  • 検出範囲
    1 µg/ml - 1000 µg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse, Rat, Human
  • 製品の概要

    ab118184, is used to determine the relative specific activity (activity and quantity) of catalase in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The assay buffer contains hydrogen peroxide which reacts with a substrate to make a luminescent product. Catalase functions rapidly to remove hydrogen peroxide from the solution and reduce the production of light. Therefore the light produced in each well is inversely proportional to the amount of catalase activity.

    After activity measurement the quantity of catalase is measured by adding to each well an anti-catalase detector antibody. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of catalase bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

  • アプリケーション適用あり: Functional Studiesmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 関連疾患Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 配列類似性Belongs to the catalase family.
  • 翻訳後修飾The N-terminus is blocked.
  • 細胞内局在Peroxisome.
  • Information by UniProt
  • 別名
    • CAT
    • CATA_HUMAN
    • Catalase
    • EC 1.11.1.6
    • MGC138422
    • MGC138424
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab118184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

Catalase Specific Activity Assay Kit 画像

  • Example sample curves demonstrating the working range of the assay for a human and cultured cell line lysate (HepG2).
  • Example standard curve for the purified human catalase sample ab91026.
  • Example sample curves demonstrating the working range of the assay for a human and cultured cell line lysate (HepG2).
  • Example standard curve for the purified human catalase sample ab91026.
  • Example relative specific activity – comparing the activity and quantity of purified erythrocyte catalase (ab91026) and catalase from human hepg2 lysate. The HepG2 sample is determined to have a higher specific activity rate.

プロトコール

Catalase Specific Activity Assay Kit (ab118184) 使用論文

ab118184 has not yet been referenced specifically in any publications.

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