製品の概要

  • 製品名
    Catalase Specific Activity Assay Kit
    Catalase キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    1 8 7.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    1 4 9.9%
  • サンプルの種類
    Cell culture extracts, Tissue Extracts
  • アッセイタイプ
    Enzyme activity
  • 検出範囲
    1 µg/ml - 1000 µg/ml
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Mouse, Rat, Human
  • 製品の概要

    ab118184, is used to determine the relative specific activity (activity and quantity) of catalase in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The assay buffer contains hydrogen peroxide which reacts with a substrate to make a luminescent product. Catalase functions rapidly to remove hydrogen peroxide from the solution and reduce the production of light. Therefore the light produced in each well is inversely proportional to the amount of catalase activity.

    After activity measurement the quantity of catalase is measured by adding to each well an anti-catalase detector antibody. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of catalase bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

  • アプリケーション
    適用あり: Functional Studiesmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 機能
    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 関連疾患
    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 配列類似性
    Belongs to the catalase family.
  • 翻訳後修飾
    The N-terminus is blocked.
  • 細胞内局在
    Peroxisome.
  • Information by UniProt
  • 別名
    • CAT
    • CATA_HUMAN
    • Catalase
    • EC 1.11.1.6
    • MGC138422
    • MGC138424
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab118184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

Catalase Specific Activity Assay Kit 画像

  • Example sample curves demonstrating the working range of the assay for a human and cultured cell line lysate (HepG2).
  • Example standard curve for the purified human catalase sample ab91026.
  • Example sample curves demonstrating the working range of the assay for a human and cultured cell line lysate (HepG2).
  • Example standard curve for the purified human catalase sample ab91026.
  • Example relative specific activity – comparing the activity and quantity of purified erythrocyte catalase (ab91026) and catalase from human hepg2 lysate. The HepG2 sample is determined to have a higher specific activity rate.

プロトコール

Catalase Specific Activity Assay Kit (ab118184) 使用論文

This product has been referenced in:
  • Padovani D  et al. Sulfheme formation during homocysteine S-oxygenation by catalase in cancers and neurodegenerative diseases. Nat Commun 7:13386 (2016). Functional Studies ; Human . Read more (PubMed: 27848965) »

See 1 Publication for this product

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