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Abcam's Catalase Assay Kit provides a highly sensitive, simple, direct and HTS-ready assay for measuring catalase activity in biological samples. In the assay, catalase first reacts with H2O2 to produce water and oxygen, the unconverted H2O2 reacts with OxiRed probe to produce a product, which can be measured at 570 nm (Colorimetric method) or at Ex/Em=535/587 nm (fluorometric method). Catalase activity is reversely proportional to the signal. The kit can detect 1 μU of catalase activity in samples.
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Catalase (EC 22.214.171.124) is a ubiquitous antioxidant enzyme that is present in nearly all living organisms. It functions to catalyze the decomposition of hydrogen peroxide (H2O2) to water and oxygen.
|Catalase Assay Buffer||NM||1 x 25ml|
|Catalase Positive Control||Blue||1 x 2µl|
|HRP (Lyophilised)||Green||1 vial|
|Hydrogen peroxide||Yellow||1 x 25µl|
|OxiRed Probe||Red||1 x 200µl|
|Stop Solution||White||1 x 1ml|
Our Abpromise guarantee covers the use of ab83464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|