Anti-Catalase 抗体 - Peroxisome Marker (ab15834)

製品の概要

  • 製品名Anti-Catalase antibody - Peroxisome Marker
    Catalase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Catalase - Peroxisome Marker
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
  • 免疫原

    Catalase (Bovine Liver)

  • ポジティブ・コントロール
    • This antibody gave a positive control in the following Tissue Lysates: Human Liver, Mouse Liver, Rat Liver, Chicken Liver

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa).

ターゲット情報

  • 機能Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 関連疾患Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 配列類似性Belongs to the catalase family.
  • 翻訳後修飾The N-terminus is blocked.
  • 細胞内局在Peroxisome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

Anti-Catalase antibody - Peroxisome Marker 画像

  • All lanes : Anti-Catalase antibody - Peroxisome Marker (ab15834) at 5 µg/ml

    Lane 1 : Catalase at 0.1 µg
    Lane 2 : Human Liver lysate at 20 µg
    Lane 3 : Mouse Liver lysate at 20 µg
    Lane 4 : Rat Liver lysate at 20 µg
    Lane 5 : Chicken Liver lysate at 20 µg

    Secondary
    Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

    Predicted band size : 60 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Catalase antibody - Peroxisome Marker (ab15834) at 5 µg/ml

    Lane 1 : Natural human Catalase protein (ab91026) at 0.1 µg
    Lane 2 : Natural human Catalase protein (ab91026) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • ICC/IF image of ab15834 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15834, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).

  • Immunofluorescence analysis of SH-SY5Y cells, staining Catalase with ab15834.

    Cells were fixed with paraformaldehyde and permeabilized with 0.25% Triton-X in PBS, followed by incubation with primary antibody on a shaker at 4°C overnight. A fluorescently labeled goat anti-rabbit IgG (diluted 1/1000) was used as the secondary antibody

Anti-Catalase antibody - Peroxisome Marker (ab15834) 使用論文

This product has been referenced in:
  • Zhou X  et al. Water restriction increases renal inner medullary manganese superoxide dismutase (MnSOD). Am J Physiol Renal Physiol 303:F674-80 (2012). Read more (PubMed: 22718889) »
  • Collins AR  et al. Myeloid deletion of nuclear factor erythroid 2-related factor 2 increases atherosclerosis and liver injury. Arterioscler Thromb Vasc Biol 32:2839-46 (2012). WB ; Mouse . Read more (PubMed: 23023374) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 1 µg
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Sample Human Cell lysate - other (Huh7 liver)
Specification Huh7 liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Tonya Colpitts

Verified customer

投稿 Nov 14 2014

Thank you for contacting us. The specific band for this antibody is typically observed around 65 kDa and the protein sequence does not suggest any isoforms or processing that would result in a specific 32 kDa band. I would suggest running a positive co...

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Thank you for your telephone enquiry yesterday afternoon. I can confirm that 10 mg of ab1877 is supplied in a vial of 1ml. I have also investigated the ICC images on the datasheets. Reviewing other antibodies that are available to catalase, it ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"