Anti-Catalase 抗体 [EP1929Y] - Peroxisome Marker (ab76024)

製品の概要

  • 製品名Anti-Catalase antibody [EP1929Y] - Peroxisome Marker
    Catalase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1929Y] to Catalase - Peroxisome Marker
  • アプリケーション適用あり: WB, ICC, Flow Cyt, IHC-Pmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminus of human Catalase

  • ポジティブ・コントロール
    • WB: HeLa cell lysate. IHC-P: human brain tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-Catalase antibody (Alexa Fluor® 488) [EP1929Y] - Peroxisome Marker (ab185041)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/20000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
    • 関連疾患Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
    • 配列類似性Belongs to the catalase family.
    • 翻訳後修飾The N-terminus is blocked.
    • 細胞内局在Peroxisome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cas1 antibody
      • CAT antibody
      • CATA_HUMAN antibody
      • Catalase antibody
      • Cs1 antibody
      • MGC138422 antibody
      • MGC138424 antibody
      see all

    Anti-Catalase antibody [EP1929Y] - Peroxisome Marker 画像

    • Overlay histogram showing HeLa cells stained with ab76024 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76024, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/20000 dilution + HeLa cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 60 kDa
      Observed band size : 60 kDa
    • Immunohistochemical staining of Catalase in paraffin embedded human normal brain tissue using ab76024 at a 1/100 dilution.

    Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) 使用論文

    This product has been referenced in:
    • Ma HP Hydrogen peroxide stimulates the epithelial sodium channel through a phosphatidylinositide 3-kinase-dependent pathway. J Biol Chem 286:32444-53 (2011). WB ; Xenopus laevis . Read more (PubMed: 21795700) »
    • Kemp K  et al. Mesenchymal stem cells restore frataxin expression and increase hydrogen peroxide scavenging enzymes in friedreich ataxia fibroblasts. PLoS One 6:e26098 (2011). WB ; Human . Read more (PubMed: 22016819) »

    See all 2 Publications for this product

    Product Wall

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (rat intestinal epithelial cells (RIE-1))
    Permeabilization Yes - 0.2% TritonX-100
    Specification rat intestinal epithelial cells (RIE-1)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative Paraformaldehyde
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    投稿 Feb 12 2016

    Application Flow Cytometry
    Sample Mouse Cell (Mouse Lewis Lung Carcinoma cells)
    Permeabilization Yes - 0.2% Triton X-100
    Gating Strategy No gating
    Specification Mouse Lewis Lung Carcinoma cells
    Preparation Cell harvesting/tissue preparation method: trypsin
    Sample buffer: Hanks’ Balanced Salt solution (HBSS)
    Fixation Paraformaldehyde
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    投稿 Jan 25 2016

    Application IHC - Wholemount
    Sample Human Tissue (Ovary adenocarcinoma)
    Specification Ovary adenocarcinoma
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    投稿 Dec 21 2015

    Application IHC - Wholemount
    Sample Mouse Tissue (Transplanted lung tumor section)
    Specification Transplanted lung tumor section
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    投稿 Dec 21 2015

    Application Western blot
    Sample African Green Monkey Cell lysate - whole cell (COS-7 cells)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Loading amount 100000 cells
    Treatment 30 nM Bortezomib for 24 hours
    Specification COS-7 cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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    投稿 Jun 24 2015

    Application Flow Cytometry
    Sample Human Cell (A549 lung cancer cells)
    Permeabilization Yes - 0.2% Triton X-100
    Gating Strategy No gating
    Specification A549 lung cancer cells
    Preparation Cell harvesting/tissue preparation method: trypsin
    Sample buffer: Hanks’ Balanced Salt solution (HBSS)
    Fixation Paraformaldehyde
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    投稿 May 15 2015

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Lewis lung cancer, FL5.12, embryonic fibroblasts)
    Gel Running Conditions Reduced Denaturing (4-20% Bis-Tris)
    Loading amount 100000 cells
    Specification Lewis lung cancer, FL5.12, embryonic fibroblasts
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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    投稿 May 11 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (mouse embryonic fibroblasts)
    Permeabilization Yes - 0.2% Triton X-100
    Specification mouse embryonic fibroblasts
    Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative Paraformaldehyde
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    投稿 May 05 2015

    Application Western blot
    Sample Human Cell lysate - whole cell (A549 lung cancer cells)
    Gel Running Conditions Reduced Denaturing (4-20% Tris-glycine)
    Loading amount 15 µg
    Specification A549 lung cancer cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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    投稿 Apr 21 2015

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