製品の概要

  • 製品名Anti-Catalase antibody
    Catalase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Catalase
  • アプリケーション適用あり: WB, ELISA, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-101 of Human Catalase with a 26 kDa tag

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 関連疾患Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 配列類似性Belongs to the catalase family.
  • 翻訳後修飾The N-terminus is blocked.
  • 細胞内局在Peroxisome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

Anti-Catalase antibody 画像



  • Predicted band size : 60 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Catalase knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab88067 observed at 60 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab88067 was shown to recognize Catalase when Catalase knockout samples were used, along with additional cross-reactive bands. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab88067 and ab181602 (loading control to GAPDH) were diluted at 1 μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Anti-Catalase antibody (ab88067) at 1 µg/ml + immunogen (recombinant fragment) at 0.2 µg

    Secondary
    Goat anti-mouse IgG at 1/5000 dilution

    Predicted band size : 60 kDa
  • Anti-Catalase antibody (ab88067) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Secondary
    Goat anti mouse IgG at 1/5000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • Overlay histogram showing HeLa cells stained with ab88067 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab88067, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Catalase antibody (ab88067) 使用論文

ab88067 has not yet been referenced specifically in any publications.

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