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Our Abpromise guarantee covers the use of ab50434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).|
|IP||Use at an assay dependent dilution. PubMed: 20923778|
|IHC-P||Use a concentration of 2 - 4 µg/ml.|
ab50434 at 2µg/ml staining Human liver tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"