製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Catalase
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human, Pig
    交差が予測される動物種: Mouse, Guinea pig, Cow, Dog, Common marmoset, Orangutan
  • 免疫原

    Synthetic peptide:

    C-DKYNAEKPKNAIHT

    , corresponding to internal sequence amino acids 498-511 of Human Catalase

  • ポジティブ・コントロール
    • Human Kidney and Liver lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IP Use at an assay dependent dilution. PubMed: 20923778
IHC-P Use a concentration of 2 - 4 µg/ml.

ターゲット情報

  • 機能
    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 関連疾患
    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 配列類似性
    Belongs to the catalase family.
  • 翻訳後修飾
    The N-terminus is blocked.
  • 細胞内局在
    Peroxisome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

画像

  • Anti-Catalase antibody (ab50434) at 1 µg/ml + Human Kidney lysate (35µg protein in RIPA buffer).

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • ab50434 at 2µg/ml staining Human liver tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used.

参考文献

This product has been referenced in:
  • Xie W  et al. Exercise training-enhanced, endothelium-dependent dilation mediated by altered regulation of BK(Ca) channels in collateral-dependent porcine coronary arterioles. Microcirculation 20:170-82 (2013). Read more (PubMed: 23002811) »
  • Habib LK  et al. Inhibitors of Catalase-Amyloid Interactions Protect Cells from {beta}-Amyloid-Induced Oxidative Stress and Toxicity. J Biol Chem 285:38933-43 (2010). IP ; Human . Read more (PubMed: 20923778) »

See all 2 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (coronary arteries and arterioles)
Loading amount
20 µg
Specification
coronary arteries and arterioles
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5µg/mL · Temperature: 37°C
Username

Dr. Wei Xie

Verified customer

投稿 Jul 13 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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