製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Catalase
  • アプリケーション
    適用あり: ICC/IF, IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Goat, Dog, Ferret, Macaque monkey, Orangutan
  • 免疫原

    Recombinant human protein purified from E.coli

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. See Abreview.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/200.
WB 1/2000. Predicted molecular weight: 60 kDa.

ターゲット情報

Anti-Catalase antibody 画像



  • Predicted band size : 60 kDa

    Western Blot analysis of cell lysates.

    Lane 1: HeLa cell lysates
    Lane 2: Jurkat cell lysates
    Lane 3: Mouse brain
    Lane 4: Rat brain

    The band marked with NS is probably non-specific.

  • ICC/IF image of ab16731 stained Hela cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16731, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Ab16731 staining human normal adrenal gland tissue. Staining is localised to intracellular compartment (peroxisomes).
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification
  • All lanes : Anti-Catalase antibody (ab16731) at 1/2000 dilution

    Lane 1 : 40ug supernatant of mouse liver homogenate
    Lane 2 : 20ug supernatant of mouse liver homogenate
    Lane 3 : 5ug supernatant of mouse liver homogenate

    Secondary
    HRP conjugated donkey anti-rabbit antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa


    Exposure time : 1 minuteThis image is courtesy of an Abreview submitted by Sandra Sobocanec on 16 March 2006.

    See Abreview

  • ab16731 at 1/200 dilution staining Catalase in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used undiluted as secondary.   

    See Abreview

  • ab16731 at a 1/200 dilution staining Catalase in mouse bone marrow cells by Immunocytochemistry/ Immunofluorescence, incubated for 9 hours at 4°C. Formalin fixed. Blocked with 2% BSA for 30 minutes at 20°C. Secondary used at 1/200 dilution polyclonal Goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green). Nuclei stained with DAPI (blue).

    See Abreview

  • ab16731 staining Catalase in mouse liver tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 before blocking with 2% BSA for 30 minutes at 200C. The sample was incubated with primary antibody (1/200) for 9 hours at 40C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

Anti-Catalase antibody (ab16731) 使用論文

This product has been referenced in:
  • Bodega G  et al. The Antioxidant Machinery of Young and Senescent Human Umbilical Vein Endothelial Cells and Their Microvesicles. Oxid Med Cell Longev 2017:7094781 (2017). WB ; Human . Read more (PubMed: 28642812) »
  • Kaczor JJ  et al. Higher oxidative stress in skeletal muscle of McArdle disease patients. Mol Genet Metab Rep 12:69-75 (2017). WB ; Human . Read more (PubMed: 28649515) »

See all 30 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Feb 01 2017

Application
Western blot
Sample
Rat Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Feb 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (synovium)
Antigen retrieval step
None
Permeabilization
No
Specification
synovium
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
zinc buffered formalin
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投稿 Jun 27 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bat Cell lysate - whole cell (Huh7 (control), P. alecto brain, fetus, kidney and)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
50 µg
Specification
Huh7 (control), P. alecto brain, fetus, kidney and
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Feb 17 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Human Cell (Human primary fibroblasts)
Specification
Human primary fibroblasts
Permeabilization
Yes - 0.2% Triton-X100
Fixative
Paraformaldehyde
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投稿 Feb 26 2014

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (4-16% gradient Tris-Gly gel)
Sample
Human Cell lysate - whole cell (TIG-1 primary fibroblasts)
Specification
TIG-1 primary fibroblasts
Treatment
33 nM catalase siRNA for 48 hrs
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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投稿 May 30 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (4-16% gradient Tris-Gly gel)
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification
Mouse embryonic fibroblasts
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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投稿 May 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Human Penile Tissue)
Loading amount
60 µg
Specification
Human Penile Tissue
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Miss. Gwen Lagoda

Verified customer

投稿 Dec 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse Lung)
Loading amount
60 µg
Specification
Mouse Lung
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Gwen Lagoda

Verified customer

投稿 Dec 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.2% Triton x-100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 25 2010

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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