製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Catalase
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Goat, Dog, Ferret, Macaque monkey, Orangutan
  • 免疫原

    Recombinant human protein purified from E.coli

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. See Abreview.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/200.
WB 1/2000. Predicted molecular weight: 60 kDa.

ターゲット情報

画像

  • Western Blot analysis of cell lysates.

    Lane 1: HeLa cell lysates
    Lane 2: Jurkat cell lysates
    Lane 3: Mouse brain
    Lane 4: Rat brain

    The band marked with NS is probably non-specific.

  • ICC/IF image of ab16731 stained Hela cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16731, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Ab16731 staining human normal adrenal gland tissue. Staining is localised to intracellular compartment (peroxisomes).
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification
  • All lanes : Anti-Catalase antibody (ab16731) at 1/2000 dilution

    Lane 1 : 40ug supernatant of mouse liver homogenate
    Lane 2 : 20ug supernatant of mouse liver homogenate
    Lane 3 : 5ug supernatant of mouse liver homogenate

    Secondary
    All lanes : HRP conjugated donkey anti-rabbit antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa


    Exposure time: 1 minute


    This image is courtesy of an Abreview submitted by Sandra Sobocanec on 16 March 2006.

    See Abreview

  • ab16731 at 1/200 dilution staining Catalase in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used undiluted as secondary.   

    See Abreview

  • ab16731 at a 1/200 dilution staining Catalase in mouse bone marrow cells by Immunocytochemistry/ Immunofluorescence, incubated for 9 hours at 4°C. Formalin fixed. Blocked with 2% BSA for 30 minutes at 20°C. Secondary used at 1/200 dilution polyclonal Goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green). Nuclei stained with DAPI (blue).

    See Abreview

  • ab16731 staining Catalase in mouse liver tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 before blocking with 2% BSA for 30 minutes at 200C. The sample was incubated with primary antibody (1/200) for 9 hours at 40C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

参考文献

This product has been referenced in:
  • Krishnan N  et al. Harnessing insulin- and leptin-induced oxidation of PTP1B for therapeutic development. Nat Commun 9:283 (2018). Read more (PubMed: 29348454) »
  • Kittivorapart J  et al. Quantitative proteomics of plasma vesicles identify novel biomarkers for hemoglobin E/ß-thalassemic patients. Blood Adv 2:95-104 (2018). Read more (PubMed: 29365317) »

See all 49 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Drosophila melanogaster Cell (hemocyte/macrophage)
Permeabilization
Yes - 0.1% Triton
Specification
hemocyte/macrophage
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Catherine Brennan

Verified customer

投稿 May 28 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (retina)
Permeabilization
No
Specification
retina
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 11 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (retina)
Gel Running Conditions
Non-reduced Denaturing (10% TGX)
Loading amount
30 µg
Specification
retina
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Minzhong Yu

Verified customer

投稿 Nov 29 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 01 2017

Application
Western blot
Sample
Rat Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (synovium)
Antigen retrieval step
None
Permeabilization
No
Specification
synovium
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
zinc buffered formalin
Username

Abcam user community

Verified customer

投稿 Jun 27 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bat Cell lysate - whole cell (Huh7 (control), P. alecto brain, fetus, kidney and)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
50 µg
Specification
Huh7 (control), P. alecto brain, fetus, kidney and
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Feb 17 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Human Cell (Human primary fibroblasts)
Specification
Human primary fibroblasts
Permeabilization
Yes - 0.2% Triton-X100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 26 2014

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (4-16% gradient Tris-Gly gel)
Sample
Human Cell lysate - whole cell (TIG-1 primary fibroblasts)
Specification
TIG-1 primary fibroblasts
Treatment
33 nM catalase siRNA for 48 hrs
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 May 30 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (4-16% gradient Tris-Gly gel)
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification
Mouse embryonic fibroblasts
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 May 30 2013

1-10 of 24 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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