Anti-Catalase 抗体 [12C2DB9] - Peroxisome Marker (ab110292)

製品の概要

  • 製品名Anti-Catalase antibody [12C2DB9] - Peroxisome Marker
    Catalase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [12C2DB9] to Catalase - Peroxisome Marker
  • アプリケーション適用あり: ICC, IP, Flow Cyt, In-Cell ELISAmore details
  • 種交差性
    交差種: Rat, Cow, Human
  • 免疫原

    Rat liver mitochondria

  • ポジティブ・コントロール
    • HeLa cells, HepG2 cells, HL60 cells, Cow heart, Rat liver
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
In-Cell ELISA Use a concentration of 4 µg/ml.

ターゲット情報

  • 機能Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 関連疾患Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 配列類似性Belongs to the catalase family.
  • 翻訳後修飾The N-terminus is blocked.
  • 細胞内局在Peroxisome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

Anti-Catalase antibody [12C2DB9] - Peroxisome Marker 画像

  • Immunocytochemical staining of Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110292 (5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody, Alexa Fluor® 488 goat anti-mouse IgG (green), was used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates mainly in peroxisomes.
  • Immunoprecipitation of Catalase using ab110292 from:
    Lane 1: HepG2 cells
    Lane 2: Cow heart sample
    Lane 3: Rat liver sample

    Predicted band size is 60 kDa.
  • Flow cytometric analysis of HL-60 cells using ab110292 at 1 µg/mL, (blue) or an isotype control (red).

Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292) 使用論文

ab110292 has not yet been referenced specifically in any publications.

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Unfortunately, the dissociation constant KD is not routinely determined and we therefore cannot provide this information on our antibodies against bovine catalase.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"