Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464)

製品の概要

  • 製品名Catalase Activity Assay Kit (Colorimetric/Fluorometric)
    Catalase キット 製品一覧
  • サンプルの種類
    Urine, Serum, Plasma, Cell Lysate, Tissue Lysate
  • アッセイタイプQuantitative
  • 全工程の試験時間
    0h 40m
  • 製品の概要

    Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids. In this assay, the catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm or fluorometrically at Ex/Em = 55/587 nm. Therefore, the catalase activity present in the sample is reversely proportional to the signal obtained. The kit can detect as little as 1 µU of catalase activity.



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  • 特記事項

    Catalase (EC 1.11.1.6) is a ubiquitous antioxidant enzyme that is present in nearly all living organisms. It catalyzes the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Catalase is a tetramer of four polypeptide chains, and contains four porphyrin heme (iron) groups that allow the enzyme to react with hydrogen peroxide. The optimum pH for human catalase is approximately pH 7, with a fairly broad maximum as the rate of reaction does not change appreciably between pH= 6.8-7.5.

  • アプリケーション適用あり: Functional Studiesmore details

法規制情報

製品の特性

  • 機能Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 関連疾患Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 配列類似性Belongs to the catalase family.
  • 翻訳後修飾The N-terminus is blocked.
  • 細胞内局在Peroxisome.
  • Information by UniProt
  • 別名
    • CAT
    • CATA_HUMAN
    • Catalase
    • EC 1.11.1.6
    • MGC138422
    • MGC138424
    see all

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab83464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Catalase Activity Assay Kit (Colorimetric/Fluorometric) 画像

  • H2O2 standard curve using ab83464.
  • Sample tests using ab83464.

プロトコール

Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) 使用論文

This product has been referenced in:
  • Ramachandran S  et al. Cyclophilin A enhances macrophage differentiation and lipid uptake in high glucose conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus. Cardiovasc Diabetol 15:152 (2016). Read more (PubMed: 27809851) »
  • Min SK  et al. Assessment of C-phycocyanin effect on astrocytes-mediated neuroprotection against oxidative brain injury using 2D and 3D astrocyte tissue model. Sci Rep 5:14418 (2015). Read more (PubMed: 26399322) »

See all 5 Publications for this product

Product Wall

Regarding the HRP  The amount of HRP once reconstituted in 200 ul is enough for 1 plate + 1 extra reaction (2 ul per reaction). This should therefore be enough for a 1 plate assay.   The 1 extra reaction amount means that if for example you want to spl...

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We have added the stop solution for ab83464 to our catalog as ab176555. A link to the product datasheet is given below:

http://www.abcam.com/stop-solution-ab176555.html


The High Control (HC) is a well that has the same volume of sample (2-78 uL) loaded as your sample wells. If you load wells with different volumes of sample, you will need to also load multiple HC wells with the same volume of sample. Prior to add...

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Gracias por contactarnos.

En el kit tiene lugar la reacción de H2O2 para producir H2O y oxigeno catalizado por la enzima catalasa. El H2O2 que queda sin reaccionar reacciona con OxiRed™ generando un producto que presenta absorban...

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Thank you for contacting us.

I can confirm that the fluorimetric detection method can be used with the 545/590 filter. Please note that the expected results could be of less quality as reading results with a 535/587 filter would be.

I...

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Thank you for contacting us. I am sorry to hear that you have had problems with our Catalase Assay Kit (ab83464)

I would like to reassure you that this kit is tested and covered by our 6 month guarantee for Tissue extracts. In the event that ...

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Thank you very much for your interest in our products.


To our knowledge, we have do not have a Catalase Assay kit in our catalog that is tested and guaranteed for plants. Since ab83464 is working by measuring the the unconverted H2O2, w...

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Thank you for your reply.


As for your samples, the dilution is extremely important and from your previous results it seems that 1:20 is best of the three you have already tested. Perhaps testing 1:25 and/or 1:30 would also be useful to ch...

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Thank you for your patience as I have investigated this issue further with the laboratory.


I can confirm that you performed the assay correctly the second time, only adding stop solution to the standards and HC ONCE.


It is n...

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The HC and standard wells already contain the stop solution. You need NOT add any more stop solution to these wells. Add the stop solution only to the sample wells in step 3 of this protocol.


Hope this information has been helpful for y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"