製品の概要

  • 製品名Anti-Caspr antibody [S65-35]
    Caspr 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [S65-35] to Caspr
  • アプリケーション適用あり: Flow Cyt, WB, IHC-P, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Fusion protein: QNHRYKGSYH TNEPKATHDS HPGGKAPLPP SGPAQAPAPT PAPTQVPTPA PAPASGPGPR DQNLPQILEE SRSE, corresponding to C terminal (cytoplasmic domain) amino acids 1308-1381 of Rat Caspr (P97846).

  • ポジティブ・コントロール
    • Rat brain lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名S65-35
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab105571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 156 kDa).
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 0.1 - 10 µg/ml.

ターゲット情報

  • 機能Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells.
  • 組織特異性Predominantly expressed in brain. Weak expression detected in ovary, pancreas, colon, lung, heart, intestine and testis.
  • 配列類似性Belongs to the neurexin family.
    Contains 2 EGF-like domains.
    Contains 1 F5/8 type C domain.
    Contains 1 fibrinogen C-terminal domain.
    Contains 4 laminin G-like domains.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Caspr antibody
    • Caspr1 antibody
    • CNTNAP antibody
    • Cntnap1 antibody
    • CNTP1_HUMAN antibody
    • Contactin associated protein 1 antibody
    • Contactin-associated protein 1 antibody
    • MHDNIV antibody
    • NCP1 antibody
    • Neurexin 4 antibody
    • Neurexin IV antibody
    • Neurexin-4 antibody
    • Nrxn4 antibody
    • p190 antibody
    • Paranodin antibody
    see all

Anti-Caspr antibody [S65-35] 画像

  • ab105571 staining Caspr in Mouse brain tissue sections by Immunohistochemistry.

  • ab105571 staining Caspr in Mouse brain tissue sections by Immunohistochemistry.

  • Anti-Caspr antibody [S65-35] (ab105571) at 1/1000 dilution + Rat brain membrane tissue lysate

    Predicted band size : 156 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab105571 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105571, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Caspr antibody [S65-35] (ab105571) 使用論文

This product has been referenced in:
  • Lourbopoulos A  et al. Changes in thyroid hormone receptors after permanent cerebral ischemia in male rats. J Mol Neurosci 54:78-91 (2014). Read more (PubMed: 24577884) »
  • Payne SC  et al. Early proliferation does not prevent the loss of oligodendrocyte progenitor cells during the chronic phase of secondary degeneration in a CNS white matter tract. PLoS One 8:e65710 (2013). IHC ; Rat . Read more (PubMed: 23776532) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab105571.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"