Anti-Caspase-9 (phospho Y153) 抗体 (ab79202)

製品の概要

  • 製品名
    Anti-Caspase-9 (phospho Y153) antibody
    Caspase-9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Caspase-9 (phospho Y153)
  • 特異性
    Detects Caspase-9 only when phosphorylated at tyrosine 153.
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-9.
    Database link: P55211

  • ポジティブ・コントロール
    • ICC: HeLa cells; WB: extracts from COLO205 cells; IHC-P: thymus tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能
    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • 組織特異性
    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • 配列類似性
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 発生段階
    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • 翻訳後修飾
    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APAF-3 antibody
    • APAF3 antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic protease Mch-6 antibody
    • Apoptotic protease-activating factor 3 antibody
    • CASP-9 antibody
    • CASP9 antibody
    • CASP9_HUMAN antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • Caspase 9 Dominant Negative antibody
    • Caspase 9c antibody
    • Caspase-9 antibody
    • Caspase-9 subunit p10 antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • ICE-LAP6 antibody
    • ICE-like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • protein phosphatase 1, regulatory subunit 56 antibody
    • RNCASP9 antibody
    see all

画像

  • Left panel: immunofluorescence analysis of HeLa cells using 1/500 ab89202. Right panel: same, after pre-incubation with the immunizing phosphopeptide.
  • Left panel: immunohistochemistry analysis of paraffin-embedded Human thymus tissue using 1/50 ab79202. Right panel: same, after pre-incubation with the immunizing phosphopeptide.
  • All lanes : Anti-Caspase-9 (phospho Y153) antibody (ab79202) at 1/500 dilution

    Lane 1 : COLO205 cell extracts
    Lane 2 : COLO205 cell extracts with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 40 kDa (possible cleavage fragment).

参考文献

ab79202 has not yet been referenced specifically in any publications.

レビューと Q&A

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Unfortunately, we do not currently know of any published references where anti-Caspase 9 (phospho Y153) antibody (ab79202) has been used. However, the antibody has been used in house to stain HeLa cells as well as...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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