Anti-Caspase-9 (phospho Y153) 抗体 (ab79202)
Key features and details
- Rabbit polyclonal to Caspase-9 (phospho Y153)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Caspase-9 (phospho Y153) antibody
Caspase-9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Caspase-9 (phospho Y153) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Caspase-9.
Database link: P55211 -
ポジティブ・コントロール
- ICC: HeLa cells; WB: extracts from COLO205 cells; IHC-P: thymus tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79202の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 46 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/500 - 1/1000.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 46 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. -
組織特異性
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. -
配列類似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
発生段階
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart. -
翻訳後修飾
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. - Information by UniProt
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参照データベース
- Entrez Gene: 842 Human
- Omim: 602234 Human
- SwissProt: P55211 Human
- Unigene: 329502 Human
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別名
- APAF-3 antibody
- APAF3 antibody
- Apoptosis related cysteine peptidase antibody
see all
画像
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All lanes : Anti-Caspase-9 (phospho Y153) antibody (ab79202) at 1/500 dilution
Lane 1 : COLO205 cell extracts
Lane 2 : COLO205 cell extracts with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Additional bands at: 40 kDa (possible cleavage fragment) -
Left panel: immunofluorescence analysis of HeLa cells using 1/500 ab89202. Right panel: same, after pre-incubation with the immunizing phosphopeptide.
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Left panel: immunohistochemistry analysis of paraffin-embedded Human thymus tissue using 1/50 ab79202. Right panel: same, after pre-incubation with the immunizing phosphopeptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab79202 は 2 報の論文で使用されています。
- Sparks NRL et al. Sidestream Smoke Extracts from Harm-Reduction and Conventional Camel Cigarettes Inhibit Osteogenic Differentiation via Oxidative Stress and Differential Activation of intrinsic Apoptotic Pathways. Antioxidants (Basel) 11:N/A (2022). PubMed: 36552682
- Gong W et al. Deletion of long noncoding RNA EFNA3 aggravates hypoxia-induced injury in PC-12 cells by upregulation of miR-101a. J Cell Biochem 120:836-847 (2019). PubMed: 30125989