特記事項（精製）Affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 46 kDa).
機能Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
組織特異性Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
配列類似性Belongs to the peptidase C14A family. Contains 1 CARD domain.
発生段階Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
翻訳後修飾Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
ab61004, at a 1/50 dilution, staining phosphoriylated Caspase-9 in human breast carcinoma tissue sections by Immunohistochemistry in the absence (left) or presence (right) of the immunizing phosphopeptide.