Caspase-9 Inhibitor Assay Kit (ab102497)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
Caspase-9 Inhibitor Assay Kit
Caspase-9 キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
全工程の試験時間
1h 30m -
製品の概要
Abcam's Caspase 9 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate LEHD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 9 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 9 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
Visit our FAQs page for tips and troubleshooting. -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K159 Caspase-9 Inhibitor Drug Screening Kit (Fluorometric). K159-100 is the same size as the 100 test size of ab102497.
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 2X Reaction Buffer 1 x 10ml Active Caspase-9 1 x 100 units Caspase Inhibitor, Z-VAD-FMK (2 mM) 1 x 10µl Caspase Substrate LEHD-AFC (1 mM) 1 x 0.5ml DTT (1 M) 1 x 100µl -
研究分野
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関連性
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex. -
別名
- APAF3
- CASP9
- Caspase 9
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関連製品
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Related Products
画像
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Titration of the caspase 9 inhibitor Z-LEHD-FMK (ab142026) (duplicates; +/- SD).
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Titration of caspase inhibitor Z-VAD-FMK (ab102497) (duplicates; +/- SD).
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Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using LEHD-AFC as caspase 9 substrate and following Caspase 9 Inhibitor Drug Detection Kit (ab102493) protocol. Lanes:
1.- Background Control: no Caspase 9, no z-VMAD-FMK
2.- Positive Inhibition Control: no Caspase 9, +z-VAD-FMK
3.- Positive Control: + Caspase 9, no z-VAD-FMK
4.- Caspase 9 + 2.5µM z-VAD-FMK
5.- Caspase 9 + 10µM z-VAD-FMK
6.- Caspase 9 + 40µM z-VAD-FMK
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab102497 は論文での使用が確認できていません。